Investigating the influence of iron on Campylobacter jejuni transcriptome in response to acid stress
Investigating the influence of iron on Campylobacter jejuni transcriptome in response to acid stress
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DOI：
10.1016/j.micpath.2019.103777
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发表时间：
2020-01-01
影响因子：
3.8
通讯作者：
Hegazy, Wael Abdel Halim
中科院分区：
文献类型：
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作者：
Askoura, Momen;Youns, Mahmoud;Hegazy, Wael Abdel Halim
C.空肠存活酸和捕获铁是C.空肠定植宿主并引起感染。在此，我们的目的是表征铁对弯曲杆菌酸反应的影响。C.在铁的存在下，空肠耐受酸应激的能力大大增强。此外，C.使用微阵列方法研究了铁富集条件下的空肠。共发现211个差异表达基因。空肠。差异表达的基因包括在21个功能组，控制弯曲杆菌的生理。C.空肠弯曲菌诱导了许多基因的表达，这些基因以前被证明对酸弯曲杆菌的存活是重要的，例如鞭毛生物发生基因和涉及细胞包膜生物发生的基因。使用RT-qPCR验证微阵列结果，其中通过两种技术获得的数据具有很大的相似性。最后，与以往的研究结果进行了比较分析，结果表明酸暴露诱导了C.空肠中未检测到的基因，如编码热休克蛋白GroEL和GroES的基因。目前的数据有助于我们理解C.空肠酸存活并因此克服这种肠道病原体的感染。
The capacity of C. jejuni to survive acid and capture iron is a requirement for C. jejuni to colonize host and cause infection. Herein, we aimed to characterize the influence of iron on Campylobacter acid response. The capacity of C. jejuni to survive acid stress was greatly enhanced in presence of iron. Moreover, the acid stimulon of C. jejuni under iron-enriched condition was investigated using the microarray approach. A total of 211 genes were differentially expressed in C. jejuni. Differentially expressed genes were included in 21 functional groups that control Campylobacter physiology. C. jejuni induced expression of many genes that were previously shown to be important for Campylobacter acid survival such as flagella biogenesis genes and genes involved in cell envelope biogenesis. The microarray results were validated using RT-qPCR where there was a great similarity in data obtained by both techniques. Finally, comparative analysis with previous studies showed that acid exposure induced expression of many genes in C. jejuni that were not detected in other studies such as genes encoding for the heat shock proteins GroEL and GroES. Current data could help us understand the mechanism of C. jejuni acid survival and consequently overcome infection by this enteric pathogen.