SERRS-based enzymatic probes for the detection of protease activity
SERRS-based enzymatic probes for the detection of protease activity
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DOI：
10.1021/ja803655h
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发表时间：
2008-09-10
影响因子：
15
通讯作者：
Graham, Duncan
中科院分区：
文献类型：
--
作者：
Ingram, Andrew;Byers, Louise;Graham, Duncan
本文报道了首次使用SerRS作为检测方法来测定蛋白酶活性。将苯丙氨酸合成地引入到苯并三氮唑偶氮染料中，可使SerRS反应“关闭”，随后暴露于蛋白酶可恢复SerRS反应。底物对一系列的蛋白酶表现出不同的反应性，并允许原位、实时地分析蛋白酶的反应性。对枯草杆菌毒素-嘉士伯酶的检测下限进行了研究，确定了该酶的检出限为50ngml−-1。
Measurement of protease activity, for the first time using SERRS as a detection method, is reported herein. Synthetic introduction of phenylalanine to a benzotriazole azo dye allows the SERRS response to be “switched off” and subsequent exposure to protease restores the SERRS response. The substrates exhibit varying reactivity for a range of proteases and allow for in situ, real-time analysis of protease reactivity. A limit of detection for one protease, Subtilisin carlsberg, was investigated and was established to be 50 ng ml−1.