The adsorption to and hydrolysis of 1,3-didecanoyl glycerol monolayers by pancreatic lipase. Effects of substrate packing density.
The adsorption to and hydrolysis of 1,3-didecanoyl glycerol monolayers by pancreatic lipase. Effects of substrate packing density.
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DOI：
10.1016/s0021-9258(19)69078-2
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发表时间：
1981-07
期刊：
影响因子：
--
通讯作者：
W. Momsen;H. Brockman
中科院分区：
文献类型：
--
作者：
W. Momsen;H. Brockman
在空气-水界面的单分子膜中测定了胰脂肪酶水解1，3-二癸酰甘油的动力学。在恒定的反应速度和表面压力的条件下，与膜相关的活性酶的量的测定表明，酶的吸附程度和吸附的酶的催化速率常数是底物包装依赖。如果将该速率常数针对从动力学和物理研究预测的反应性底物的浓度进行校正（Momsen，W. E、和Brockman，H. L.（1979）J.Biol.Chem.254，8855-8860），2.5 +/-0.5 X 10（12）（S.E.）cm ~ 2/（mol.s）的二级速率常数。这种恒定性支持的假设，包装依赖的构象分布的基板头组是一个因素，在调节脂解。
The kinetics of hydrolysis of 1,3-didecanoyl glycerol by pancreatic lipase were measured in monomolecular films at the air-water interface. Determination of the quantity of active enzyme associated with the film under conditions of constant reaction velocity and surface pressure showed that both the extent of enzyme adsorption and the catalytic rate constant of the adsorbed enzyme were substrate packing dependent. If this rate constant was corrected for the concentration of reactive substrate predicted from kinetic and physical studies (Momsen, W. E., and Brockman, H. L. (1979) J. Biol. Chem. 254, 8855-8860), a constant value of 2.5 +/- 0.5 X 10(12) (S.E.) cm2/(mol.s) was obtained for the second order rate constant. This constancy supports the hypothesis that a packing dependent conformational distribution of substrate head groups is a factor in the regulation of lipolysis.