Distribution and characterization of vanilloid receptors in the rat stomach
Distribution and characterization of vanilloid receptors in the rat stomach
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DOI：
10.1016/s0304-3940(01)02021-3
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发表时间：
2001-08-17
影响因子：
2.5
通讯作者：
Matsuura, N
中科院分区：
文献类型：
--
作者：
Nozawa, Y;Nishihara, K;Matsuura, N
克隆的香草素受体-1（VR 1）被认为是质子、有害热和香草素的共同分子靶点。VR 1在背根、三叉神经和结状神经节中的存在已被确定，但在肠道中尚不清楚，尽管VR 1可能对胃粘膜稳态很重要。本研究用一种抗体和一种放射性配体显示了辣椒素受体（vanilloid receptor，VR）在大鼠胃中的分布，并对其进行了表征。VR 1免疫阳性神经末梢主要分布在增殖区的粘液颈细胞和粘膜下层的血管周围。用[H-3]-树脂毒素（RTX）的放射受体分析表明，粘膜的膜组分中存在高亲和力和单类结合位点。辣椒素完全抑制[H-3]-RTX的特异性结合。VR 1的免疫反应性和受体密度的[H-3]-RTX结合位点显着减少的应用辣椒素在大鼠粘膜中的时间延长。我们的研究结果表明，VRS在大鼠胃中表达，并表明它们可能通过增加细胞增殖和血流量参与粘膜保护。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
The cloned vanilloid receptor-1 (VR1) is recognized as a common molecular target for protons, noxious heat, and vanilloids. The presence of VR1 in the dorsal root, trigeminal, and nodose ganglia has been firmly established, but it is unclear in the gut, despite this VR1 may be important for gastric mucosal homeostasis. In this study we used an antibody and a radioligand to show the distribution of vanilloid receptors (VRs) in rat stomach and to characterize it. The deaf-ferentiation of capsaicin-sensitive nerves in rats was induced by consecutive injections of capsaicin. VR1-immunopositive nerve endings were predominantly found in the mucous neck cells of the proliferation zone, and around blood vessels in the submucosa. Radioreceptor assay using [H-3]-resiniferatoxin (RTX) revealed the existence of high affinity and single-class binding site in the membrane fractions of the mucosa. Capsaicin completely inhibited the specific binding of [H-3]-RTX. Both the VR1 immunoreactivity and the receptor density of [H-3]-RTX binding sites significantly reduced by the application of capsaicin for prolonged periods of time in the mucosa of rats. Our results indicate that VRS are expressed in the rat stomach, and suggest that they may be involved in mucosal protection by increasing cell proliferation and blood flow. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.