THE UNRELATED SURFACE-PROTEINS ACTA OF LISTERIA-MONOCYTOGENES AND ICSA OF SHIGELLA-FLEXNERI ARE SUFFICIENT TO CONFER ACTIN-BASED MOTILITY ON LISTERIA-INNOCUA AND ESCHERICHIA-COLI RESPECTIVELY
THE UNRELATED SURFACE-PROTEINS ACTA OF LISTERIA-MONOCYTOGENES AND ICSA OF SHIGELLA-FLEXNERI ARE SUFFICIENT TO CONFER ACTIN-BASED MOTILITY ON LISTERIA-INNOCUA AND ESCHERICHIA-COLI RESPECTIVELY
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DOI：
10.1111/j.1365-2958.1995.mmi_18030413.x
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发表时间：
1995-11-01
影响因子：
3.6
通讯作者：
COSSART, P
中科院分区：
文献类型：
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作者：
KOCKS, C;MARCHAND, JB;COSSART, P
单核细胞增生李斯特菌（Listeria monocytogenes）和福氏志贺菌（Shigella flexneri）是两种不相关的兼性胞内病原菌，它们通过基于在细菌的一极连续肌动蛋白组装的相似的胞内运动模式在细胞间传播。单核细胞增多症和IcsA（VirG）表面蛋白在S. flexneri、ActA和IcsA没有序列同源性。为了评估这两种蛋白质在产生基于肌动蛋白的运动中的作用，我们在两种非肌动蛋白聚合的非致病性细菌物种，无害李斯特菌和大肠杆菌的遗传背景下表达了它们。在没有任何额外的细菌致病性决定因素的情况下，这两种蛋白质诱导了非洲爪蟾卵细胞质提取物中细菌的肌动蛋白组装和推进，如特征性肌动蛋白彗星尾的形成所示。E.表达IcsA的大肠杆菌比李斯特菌移动快约两倍，并显示出更长的肌动蛋白尾。然而，肌动蛋白动力学（肌动蛋白丝分布和丝半衰期）在IcsA和ActA诱导的肌动蛋白尾中相似，表明通过使用不相关的表面分子，李斯特菌可以表达IcsA。单核细胞增多症和S.弗氏杆菌通过与相同的宿主细胞骨架组分相互作用或通过干扰相同的宿主细胞信号转导途径而在细胞内移动。
Listeria monocytogenes and Shigella flexneri are two unrelated facultative intracellular pathogens which spread from cell to cell by using a similar mode of intracellular movement based on continuous actin assembly at one pole of the bacterium, This process requires the asymmetrical expression of the ActA surface protein in L. monocytogenes and the IcsA (VirG) surface protein in S. flexneri, ActA and IcsA share no sequence homology. To assess the role of the two proteins in the generation of actin-based movement, we expressed them in the genetic context of two nonactin polymerizing, non-pathogenic bacterial species, Listeria innocua and Escherichia coli, In the absence of any additional bacterial pathogenicity determinants, both proteins induced actin assembly and propulsion of the bacteria in cytoplasmic extracts from Xenopus eggs, as visualized by the formation of characteristic actin comet tails. E. coli expressing IcsA moved about two times faster than Listeria and displayed longer actin tails, However, actin dynamics (actin filament distribution and filament half-lives) were similar in IcsA- and ActA-induced actin tails suggesting that by using unrelated surface molecules, L. monocytogenes and S. flexneri move intracellularly by interacting with the same host cytoskeleton components or by interfering with the same host cell signal transduction pathway.