Distribution of the ACME-arcA gene among methicillin-resistant Staphylococcus aureus from England and Wales
Distribution of the ACME-arcA gene among methicillin-resistant Staphylococcus aureus from England and Wales
复制标题
DOI：
10.1093/jac/dkm422
复制
发表时间：
2008-01-01
影响因子：
5.2
通讯作者：
Kearns, Angela M.
中科院分区：
文献类型：
--
作者：
Ellington, Matthew J.;Yearwood, Lianne;Kearns, Angela M.
背景资料：ST 8-SCCmecIVa（USA 300）耐甲氧西林金黄色葡萄球菌（MRSA）克隆可携带精氨酸分解代谢移动的元件（ACME）。ACME内的arc基因簇可能作为毒力或菌株存活因子起作用。我们确定了来自英格兰和威尔士各地的遗传多样性MRSA中ACME相关arcA基因的分布。方法：将来自英格兰和威尔士葡萄球菌参考实验室的不同遗传类型的MRSA分离株（n = 203）进行了ACME相关arcA基因的检测。ACME-arcA阳性菌株的特征在于毒素基因谱，PFGE和spa序列分型。结果：在17株分离株中检测到ACME-arcA基因。12个与已知的USA 300谱系的ST 8-MRSA-SCCmecIVa分离株通过脉冲分型相关，并且对苯唑西林耐药，具有可变的环丙沙星和红霉素耐药。在USA 300谱系之外，其余5个ACME-arcA分离株中的4个与ST 97-MRSA-SCCmecV密切相关，Panton-Valentine杀白细胞素（PVL）阴性，对苯唑西林耐药，对红霉素、环丙沙星、克林霉素、庆大霉素、四环素和夫西地酸耐药。其余分离株为ST 1，PVL阳性，对夫西地酸和苯唑西林耐药。17株中有13株与皮肤和软组织infections.Conclusions：ACME-arcA在不同MRSA类型中的检测突出了编码ACME-arcA基因的元件的移动性。编码ACME-arcA的MRSA菌株类型和耐药谱的多样性是引起公共卫生关注的原因，需要持续监测和密切监测。
Background: The ST8-SCCmecIVa (USA300) methicillin-resistant Staphylococcus aureus (MRSA) clone can harbour the arginine catabolic mobile element (ACME). The arc gene cluster within the ACME may function as a virulence or strain survival factor. We determined the distribution of the ACME-associated arcA gene among genetically diverse MRSA from around England and Wales.Methods: MRSA isolates (n = 203) of diverse genetic types, referred to the England and Wales Staphylococcus reference laboratory, were tested for the presence of the ACME-arcA gene. ACME-arcA-positive isolates were characterized by toxin gene profiling, PFGE and spa sequence typing. MICs of a range of antimicrobials were also determined.Results: The ACME-arcA gene was detected in 17 isolates. Twelve were related to known ST8-MRSA-SCCmecIVa isolates of the USA300 lineage by pulsotype and were resistant to oxacillin, with variable ciprofloxacin and erythromycin resistance. Outside the USA300 lineage, four of the remaining five ACME-arcA isolates were closely related ST97-MRSA-SCCmecV, Panton-Valentine leucocidin (PVL)-negative, resistant to oxacillin and variously resistant to erythromycin, ciprofloxacin, clindamycin, gentamicin, tetracycline and fusidic acid. The remaining isolate was ST1, PVL-positive and resistant to fusidic acid as well as oxacillin. Thirteen out of the 17 isolates were associated with skin and soft tissue infections.Conclusions: The detection of ACME-arcA in diverse MRSA types highlights the mobility of the elements encoding ACME-arcA genes. The diversity of strain types and resistance profiles among ACME-arcA-encoding MRSA is a cause for public-health concern and demands continued surveillance and close monitoring.