Remarkable Antibacterial Activity of Reduced Graphene Oxide Functionalized by Copper Ions
Remarkable Antibacterial Activity of Reduced Graphene Oxide Functionalized by Copper Ions
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DOI：
10.1002/adfm.202008018
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发表时间：
2020-09
影响因子：
19
通讯作者：
Y. Tu;Pei Li;Jiajia Sun;Jie Jiang;Fangfang Dai;Chengzhang Li;Yuanyan Wu;Liang Chen;Guosheng Shi;Yanwen Tan;Haiping Fang
中科院分区：
文献类型：
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作者：
Y. Tu;Pei Li;Jiajia Sun;Jie Jiang;Fangfang Dai;Chengzhang Li;Yuanyan Wu;Liang Chen;Guosheng Shi;Yanwen Tan;Haiping Fang
尽管长期以来人们一直在努力探索抗菌剂或药物，但在增强抗菌活性的同时尽量减少对环境的毒性仍然是一个挑战。在这里，实验表明，还原氧化石墨烯（rGO）通过铜离子的功能表现出选择性抗菌活性，其活性明显强于还原氧化石墨烯本身，并且对哺乳动物细胞没有毒性。值得注意的是，这种抗菌活性比其周围铜离子的活性高两个数量级。结果表明，还原氧化石墨烯通过阳离子-π相互作用被功能化，大量吸附铜离子形成还原氧化石墨烯-铜复合材料，使周围铜离子浓度极低（小于≈0.5µm）。还原氧化石墨烯上的铜离子带正电荷，与带负电荷的细菌细胞发生强烈的相互作用，选择性地获得抗菌活性，而还原氧化石墨烯不仅具有铜离子快速传递和大量组装到细菌细胞上的功能，而且还能导致铜离子的价态从Cu2+转变为Cu+，从而大大增强了抗菌活性。值得注意的是，通过与铜离子的阳离子-π相互作用，氧化石墨烯的功能同样可以达到杀藻活性，但不会对中性带电的哺乳动物细胞产生细胞毒性。
Despite long‐term efforts for exploring antibacterial agents or drugs, potentiating antibacterial activity and meanwhile minimizing toxicity to the environment remains a challenge. Here, it is experimentally shown that the functionality of reduced graphene oxide (rGO) through copper ions displays selective antibacterial activity that is significantly stronger than that of rGO itself and no toxicity to mammalian cells. Remarkably, this antibacterial activity is two‐orders‐of‐magnitude greater than the activity of its surrounding copper ions. It is demonstrated that rGO is functionalized through the cation–π interaction to massively adsorb copper ions to form a rGO–copper composite and result in an extremely low concentration level of surrounding copper ions (less than ≈0.5 µm). These copper ions on rGO are positively charged and strongly interact with negatively charged bacterial cells to selectively achieve antibacterial activity, while rGO exhibits the functionality to not only actuate rapid delivery of copper ions and massive assembly onto bacterial cells but also result in the valence shift in the copper ions from Cu2+ into Cu+, which greatly enhances the antibacterial activity. Notably, this rGO functionality through cation–π interaction with copper ions can similarly achieve algaecidal activity but does not exert cytotoxicity against neutrally charged mammalian cells.