Endogenous retinoids in rat epididymal tissue and rat and human spermatozoa.
Endogenous retinoids in rat epididymal tissue and rat and human spermatozoa.
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大鼠附睾组织以及大鼠和人类精子中的内源性类维生素A。
DOI：
10.1095/biolreprod48.2.235
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发表时间：
1993
影响因子：
3.6
通讯作者：
Ong,DE
中科院分区：
文献类型：
--
作者：
Pappas,RS;Newcomer,ME;Ong,DE
最近的工作表明，高水平的维甲酸结合蛋白在大鼠附睾，和内腔维甲酸结合蛋白已被纯化。这些结果表明，维生素A可能参与精子在附睾中的成熟。我们进一步解决了这个问题，定量视黄醇，视黄酯，视黄酸异构体从灌注附睾组织，从大鼠睾丸和附睾精子，并从人类射精spermacle.HPLC显示维生素A水平是在头部高于体或尾部组织。维甲酸和9-顺式维甲酸被认为是从最低水平的头部到最高的尾部。精子从附睾头和丰富的睾丸精子被发现有更高的维生素A水平比精子从附睾体或尾部。精子视黄酯的酰基取代基与整个附睾中的相似，而精子远端段的视黄酯的数量减少，人类射精精子中的视黄酯棕榈酸酯和硬脂酸酯含量较高。维生素A和视黄酸在人类精子中仅能微量检测到，类维生素A在成熟精子中的保留表明维生素A在精子生殖生理学中的作用超过了附睾阶段。
Recent work has demonstrated high levels of retinoid binding proteins in rat epididymis, and a lumenal retinoic acid binding protein has been purified. These findings suggested that vitamin A may be involved in spermatozoal maturation in the epididymis. We further addressed this question by quantifying retinol, retinyl esters, and retinoic acid isomers from perfused epididymal tissue, from rat testicular and epididymal spermatozoa, and from human ejaculate sperm.HPLC showed vitamin A levels to be higher in caput than in corpus or cauda tissue. Retinoic acid and 9-cis-retinoicacid were found to be graded from lowest levels in caput to highest in cauda.Spermatozoa from caput epididymidis and enriched testicular spermatozoa were found to have higher levels of vitamin A than did spermatozoa from corpus or cauda epididymidis. Spermatozoal retinyl esters had acyl substituents similar to those seen in whole epididymis, and diminished in quantity in sperm from distal segments.Human ejaculate sperm were found to retain high levels of retinyl palmitate and stearate. Retinol and retinoic acid were only marginally detectable in human sperm.Retention of retinoids in mature spermatozoa suggests roles for vitamin A in spermatozoal reproductive physiology beyond the epididymal stage.