Accessory limb induction on flank region and its muscle regulation in axolotl
Accessory limb induction on flank region and its muscle regulation in axolotl
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DOI：
10.1002/dvdy.23984
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发表时间：
2013-08-01
影响因子：
2.5
通讯作者：
Satoh, Akira
中科院分区：
文献类型：
--
作者：
Hirata, Ayako;Makanae, Aki;Satoh, Akira
背景：有尾目两栖动物具有很强的再生能力，这一点已被研究很久。近年来，一种新的实验系统--副肢模型的发展成为两栖动物肢体再生研究的新选择。虽然副肢模型有许多优点，但在一些具体的分析中，如研究肌肉起源，需要改进。为此，尝试在非肢体区域进行辅助肢体诱导。结果：通过神经偏移和肢体皮肤移植，在非肢体区域（侧腹）诱导副肢体是可能的。维甲酸注射液可提高诱导率。诱导肢体具有与正常肢体相同的组织背景。还观察到大量的肌细胞。推测肌细胞来源于侧腹肌组织，因为肢体肌细胞是迁移性细胞群，并且副肢体是在远离原始肢体的地方诱导的。我们还发现，肌肉细胞的迁移是由Hgf/cMet信号调节，在其他脊椎动物。结论：即使在非肢体侧腹区域，副肢体诱导也是可能的。侧翼诱导的肢体将有助于进一步分析肢体再生，特别是迁移细胞群，如肌肉细胞。(C)2013 Wiley Periodicals，Inc.
Background: Urodele amphibians have high regeneration capability that has been studied for a long time. Recently, a new experimental system called the accessory limb model was developed and becomes alternative choice for amphibian limb regeneration study. Although the accessory limb model has many advantages, an improvement was needed for some specific analysis, such as studying muscle origin. For that purpose, an accessory limb induction on nonlimb regions was attempted. Results: Accessory limb induction on a nonlimb region (flank) was possible by nerve deviation and limb skin grafting. Retinoic acid injections improved the induction rate. The induced limb possessed the same tissue context as a normal limb. Muscle cells were also abundantly observed. It is speculated that the muscle cells are derived from flank muscle tissues, because limb muscle cells are a migratory cell population and the accessory limb was induced apart from the original limb. We also found that migration of the muscle cells was regulated by Hgf/cMet signaling as in other vertebrates. Conclusions: Accessory limb induction was possible even in the nonlimb flank region. The flank-induced limb would be useful for further analysis of limb regeneration, especially for migratory cell populations such as muscle cells. (C) 2013 Wiley Periodicals, Inc.