Development of a real-time bioprocess monitoring method for docosahexaenoic acid production by Schizochytrium sp.
Development of a real-time bioprocess monitoring method for docosahexaenoic acid production by Schizochytrium sp.
复制标题
开发裂殖壶菌生产二十二碳六烯酸的实时生物过程监测方法。
DOI：
10.1016/j.biortech.2016.05.044
复制
发表时间：
2016-09-01
影响因子：
11.4
通讯作者：
Huang, He
中科院分区：
文献类型：
--
作者：
Guo, Dong-Sheng;Ji, Xiao-Jun;Huang, He
摄氧率(OUR)和呼吸商(RQ)是裂殖酵母生产二十二碳六烯酸(DHA)的关键呼吸参数。HX-308在溶氧限制条件下。为了研究OUR和RQ与培养状态的关系，在50型L生物反应器中进行了三种不同曝气量的独立培养。我们发现OUR与曝气率呈正相关，这反映了每个培养物的供氧水平。最大生物量为124.5 g/L，是在最高UE下获得的。DHA含量与RQ值高度相关，RQ值最高时DHA含量最高(占总脂肪酸的44.85%，w/w)，这表明聚酮合成酶途径更活跃。OUR和RQ反映了微生物的生理状态，可作为DHA发酵的协同实时生物过程监测参数。(C)2016爱思唯尔有限公司。保留所有权利。
Oxygen uptake rate (OUR) and respiratory quotient (RQ) are key respiratory parameters for docosahexaenoic acid (DHA) production by Schizochytrium sp. HX-308 under dissolved oxygen limited conditions. To investigate the relationship of OUR and RQ with culture status, three independent cultures with different aeration rates were performed in a 50 L bioreactor. OUR was found to be positively correlated with the aeration rate, which reflected the oxygen supply level in each culture. The highest biomass, reaching 124.5 g/L, was achieved under the highest OUR. DHA content was found to be highly correlated with the RQ value, and the highest DHA content (44.85% in total fatty acids, w/w) was achieved in the highest RQ level, which implies that the polyketide synthase pathway was more active. OUR and RQ, which reflect the physiological state of microorganisms, are suggested as synergistic real-time bioprocess monitoring parameters for DHA fermentation. (C) 2016 Elsevier Ltd. All rights reserved.