Drug-release controlling nanoparticles with field-dependent spin-correlated radical pair system
Drug-release controlling nanoparticles with field-dependent spin-correlated radical pair system
复制标题
具有场依赖性自旋相关自由基对系统的药物释放控制纳米颗粒
DOI：
10.1016/j.jmmm.2020.166558
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发表时间：
2020
影响因子：
2.7
通讯作者：
Takashi Tadokoro
中科院分区：
文献类型：
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作者：
Hidenori Nakagawa;Takashi Tadokoro
磁场效应在自旋相关自由基对机制上的应用是脂质体药物释放技术的新途径。以7种磷脂为原料制备了不同类型的纳米脂质体，并对其在可能的磁控条件下的释药电位进行了研究。使用永磁体（0.25 T）或空心线圈系统（60 mT）暴露于静态磁场中。使用原始的风冷紫外- led照射系统对含氟酰胺的脂质体纳米颗粒进行光照射。在静电场作用下，脂质体膜中的氟他胺被325 nm的发射波长激发。激发后不久，连续测量吸光度在314、260和370 nm处的光谱变化。此外，我们还利用a31P核磁共振技术研究了磁场对模型膜系统渗透率的影响。根据我们的总体结果，场依赖性的差异可以肯定地解释为形成单线态自由基对衍生的笼状产物，这可能在脂质体双层结构中具有额外的空间阻碍因素。用脂质体膜磷脂中不饱和双键的数目对实验结果进行了讨论。具有反应场依赖自由基对机制的脂质体系统是一个有前景的新研究领域。
The use of magnetic field effects on spin-correlated radical pair mechanisms is a new approach for applications to liposomal drug-release technologies. We prepared various types of liposomal nanoparticles from seven kinds of phospholipids, and carried out research into the drug-release potentials of the nanoparticles equipped with possible magnetic controls. Exposures to static magnetic fields were performed using a permanent magnet (0.25 T) or an air-cored coil system (60 mT). Photoirradiations of flutamide-containing liposomal nanoparticles were carried out using an original air-cooled UV-LED irradiation system. Under exposure to the static field, flutamide in the liposomal membrane was excited by an emission wavelength of 325 nm. Soon after the excitation was achieved, the spectral changes with absorbance at 314, 260, and 370 nm were measured continuously. Moreover, we investigated the magnetic field effects on the permeability of the model membrane systems, using a31P NMR technique. According to our overall results, differences in the field dependence can be surely explained by the formation of a singlet radical pair-deriving cage product, which might have an additional steric hindering factor in the liposomal bilayer structure. The experimental result is discussed in terms of the number of unsaturated double bonds in phospholipids of liposomal membranes. Our liposomal system with reaction field-dependent radical pair mechanisms is a promising new area of research.