Platelet compatible blood filtration fabrics using a phosphorylcholine polymer having high surface mobility
Platelet compatible blood filtration fabrics using a phosphorylcholine polymer having high surface mobility
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DOI：
10.1016/s0142-9612(03)00212-6
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发表时间：
2003-09-01
期刊：
影响因子：
14
通讯作者：
Ishihara, K
中科院分区：
文献类型：
--
作者：
Iwasaki, Y;Yamasaki, A;Ishihara, K
为了获得一种新型的用于血液过滤设备涂层的聚合物，该聚合物在干燥条件下与血液接触时可以减少血小板的黏附和激活，设计了一种具有高迁移率的聚合物侧链的磷胆碱聚合物。该聚合物具有2-甲基丙烯酰氧基乙氧乙基磷酰胆碱单元(PME02B)，在磷酰胆碱基和主链之间具有二环氧乙烷链。用X-射线光电子能谱分析了水溶液中磷烯醇基的表面密度和取向。在PME02B表面，即使在空气气氛中，磷酰胆碱基团的表面密度也远高于理论值。与2-甲基丙烯酰氧乙基磷酰胆碱聚合物(PMB)相比，PME02B的水合平衡时间更短。用聚对苯二甲酸乙二醇酯纤维制成的无纺布和涂覆磷胆碱聚合物的无纺布对血小板的活化和粘附性进行了评价。即使当血小板通过PME02B涂层织物而没有预水合时，洗脱出的血小板的活性与天然血小板的活性相似。此外，在织物上没有观察到贴壁细胞。另一方面，血小板附着在PET织物和涂有PMB的织物上。基于这些结果，我们得出结论，聚合物链的较高迁移率对于减少与血小板的相互作用非常重要。(C)2003爱思唯尔科学有限公司。保留所有权利。
obtain a novel polymer for coating on blood filtration devices, which can reduce platelet adhesion and activation when the polymer is in contact with blood under a dry condition, a phosphorylcholine polymer with high mobility of the polymer side chain was designed. The polymer possesses 2-methacryloyloxyethoxyethyl phosphorylcholine unit (PME02B) having a diethylene oxide chain between the phosphorylcholine group and the backbone. The surface density of the phosphoryleholine groups and their orientation under aqueous conditions were analyzed with an X-ray photoelectron spectroscope. On the PME02B surface, the surface density of phosphorylcholine groups was much higher than that of the theoretical value even when the surface was in air atmosphere. The period for equilibrating the surface of PME02B by hydration was shorter than that of the 2-methacryloyloxyethyl phosphorylcholine polymer (PMB). The mobility of the polymer chain with hydration was remarkably improved with the addition of a diethylene oxide chain as a bridging unit.The platelet activation and adhesion were evaluated using a non-woven fabric made from poly(ethylene terephthalate) fibers and that coated with these phosphorylcholine polymers. Even when the platelets were passed through the PME02B-coated fabric without prehydration, the activity of the platelets eluted was similar to that of native platelets. Moreover, adherent cells were not observed on the fabric. On the other hand, the platelets adhered to the PET fabric and to that coated with PMB. Based on these results, we concluded that the higher mobility of the polymer chain is very important to reduce interactions with platelets. (C) 2003 Elsevier Science Ltd. All rights reserved.