Accumulation of orally administered quercetin in brain tissue and its antioxidative effects in rats
Accumulation of orally administered quercetin in brain tissue and its antioxidative effects in rats
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DOI：
10.1016/j.freeradbiomed.2011.06.017
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发表时间：
2011-10-01
影响因子：
7.4
通讯作者：
Terao, Junji
中科院分区：
文献类型：
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作者：
Ishisaka, Akari;Ichikawa, Satomi;Terao, Junji
槲皮素广泛分布于蔬菜和草药中，并被认为是一种神经保护剂。在这里，我们证明了槲皮素可以积累到足以发挥生物活性，在大鼠脑组织。用β-葡萄糖醛酸苷酶/硫酸酯酶处理没有可检测的血红蛋白污染物的灌注大鼠脑匀浆，并使用高效液相色谱法（HPLC）和三种不同的检测方法分析释放的槲皮素及其甲基化形式。使用HPLC-UV和HPLC电化学检测在槲皮素给药大鼠的脑中检测到槲皮素和甲基化形式，并使用HPLC-串联质谱法进一步鉴定。口服槲皮素（50 mg/kg体重）可减轻慢性强迫游泳大鼠海马和纹状体中氧化应激的增加。槲皮素衍生物进入脑组织的可能的运输在体外复制通过使用大鼠脑毛细血管内皮细胞系，血脑屏障的模型。这些结果表明，槲皮素可能是一种有效的营养素，可以进入大脑并保护其免受与氧化应激相关的疾病的影响。(C)2011 Elsevier Inc. All rights reserved.
Quercetin is widely distributed in vegetables and herbs and has been suggested to act as a neuroprotective agent. Here, we demonstrate that quercetin can accumulate enough to exert biological activity in rat brain tissues. Homogenates of perfused rat brain without detectable hemoglobin contaminants were treated with beta-glucuronidase/sulfatase and the released quercetin and its methylated form were analyzed using high-performance liquid chromatography (HPLC) with three different detection methods. Both quercetin and the methylated form were detected in the brain of quercetin-administered rats using HPLC-UV and HPLC with electrochemical detection and were further identified using HPLC-tandem mass spectrometry. Oral administration of quercetin (50 mg/kg body wt) attenuated the increased oxidative stress in the hippocampus and striatum of rats exposed to chronic forced swimming. The possible transport of quercetin derivatives into the brain tissue was reproduced in vitro by using a rat brain capillary endothelial cell line, a model of the blood-brain barrier. These results show that quercetin could be a potent nutrient that can access the brain and protect it from disorders associated with oxidative stress. (C) 2011 Elsevier Inc. All rights reserved.