Multiple particle tracking microrheological characterization: Fundamentals, emerging techniques and applications
Multiple particle tracking microrheological characterization: Fundamentals, emerging techniques and applications
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DOI：
10.1063/5.0006122
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发表时间：
2020-05-29
影响因子：
3.2
通讯作者：
Schultz, Kelly M.
中科院分区：
文献类型：
--
作者：
McGlynn, John A.;Wu, Nan;Schultz, Kelly M.
多粒子跟踪微观流变学(MPT)是一种被动的微观流变学技术，它测量嵌入样品中的探针粒子的布朗运动，以表征材料的流变特性。MPT是一种强大的工具，可以在低模数范围内量化材料的流变性，同时只需要少量的样品和使用视频显微镜进行相对简单的数据采集。MPT定量表征了时空流变性，特别适合于研究具有复杂微环境的演化材料。MPT已经扩展了对各种材料的研究，包括生物膜、胶体凝胶、水凝胶、刺激响应材料和细胞生物材料。本教程的目的是总结基本原理，说明其多功能性，并重点介绍MPT的最新进展。在每个应用中，我们将重点介绍MPT如何在收集流变特性方面具有独特的定位，这是其他流变学表征技术难以(如果不是不可能)实现的，并将重点介绍MPT如何用于补充其他测量技术。本教程应该为研究人员提供使用MPT所需的基本基础和技能，并开发新的MPT技术来表征材料的独特应用。
Multiple particle tracking microrheology (MPT) is a passive microrheological technique that measures the Brownian motion of probe particles embedded in a sample to characterize material rheological properties. MPT is a powerful tool that quantifies material rheology in the low moduli range while requiring only small sample volumes and relatively simple data acquisition using video microscopy. MPT quantitatively characterizes spatiotemporal rheological properties and is particularly well suited for the investigation of evolving materials with complex microenvironments. MPT has expanded the study of a variety of materials including biofilms, colloidal gels, hydrogels, stimuli-responsive materials, and cell-laden biomaterials. The aim of this Tutorial is to summarize the fundamentals, illustrate the versatility, and highlight recent advances in MPT. In each application, we will highlight how MPT is uniquely positioned to gather rheological properties, which would be difficult, if not impossible, to attain with other rheological characterization techniques and highlight how MPT can be used to supplement other measurement techniques. This Tutorial should provide researchers with the fundamental basis and skills needed to use MPT and develop new MPT techniques to characterize materials for their unique applications.