Systematic characterization of seminal plasma piRNAs as molecular biomarkers for male infertility.
Systematic characterization of seminal plasma piRNAs as molecular biomarkers for male infertility.
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精浆 piRNA 作为男性不育分子生物标志物的系统表征
DOI：
10.1038/srep24229
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发表时间：
2016-04-12
影响因子：
4.6
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Hong Y;Wang C;Fu Z;Liang H;Zhang S;Lu M;Sun W;Ye C;Zhang CY;Zen K;Shi L;Zhang C;Chen X
尽管piwi相互作用RNAs(PiRNAs)在精子发生中起着关键作用，但对不育男性精浆中的piRNAs知之甚少。在这项研究中，我们系统地研究了不育男性精浆piRNAs的分布，以确定在不育过程中发生变化的piRNAs，并评价其诊断价值。精浆标本取自211例不育患者(弱精子症和无精子症)和91例正常生育者。高通量测序技术被用于筛选来自健康对照组和不育患者的精浆样本中的piRNA图谱。结果发现，与对照组相比，不孕症患者组有61个piRNAs发生了明显的改变。接下来，在训练和验证集中进行定量RT-PCR分析，以测量和确认改变的piRNAs的浓度。结果发现，与健康对照组相比，不育患者精浆中的5个piRNAs显著减少。ROC曲线分析和Risk Score分析表明，这5个piRNAs对弱精子症和无精子症患者与健康对照的诊断潜力很高。总而言之，这项研究确定了一组能够准确区分生育和不育男性的piRNA。这一发现可能为不孕症的发展提供病理生理学线索。
Although piwi-interacting RNAs (piRNAs) play pivotal roles in spermatogenesis, little is known about piRNAs in the seminal plasma of infertile males. In this study, we systematically investigated the profiles of seminal plasma piRNAs in infertile males to identify piRNAs that are altered during infertility and evaluate their diagnostic value. Seminal plasma samples were obtained from 211 infertile patients (asthenozoospermia and azoospermia) and 91 fertile controls. High-throughput sequencing technology was employed to screen piRNA profiles in seminal plasma samples pooled from healthy controls and infertile patients. The results identified 61 markedly altered piRNAs in infertile patient groups compared with control group. Next, a quantitative RT-PCR assay was conducted in the training and validation sets to measure and confirm the concentrations of altered piRNAs. The results identified a panel of 5 piRNAs that were significantly decreased in seminal plasma of infertile patients compared with healthy controls. ROC curve analysis and risk score analysis revealed that the diagnostic potential of these 5 piRNAs to distinguish asthenozoospermic and azoospermic individuals from healthy controls was high. In summary, this study identifies a panel of piRNAs that can accurately distinguish fertile from infertile males. This finding may provide pathophysiological clues about the development of infertility.