Cell line provenance
Cell line provenance
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DOI：
10.1023/a:1022949730029
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发表时间：
2002-01-01
期刊：
影响因子：
2.2
通讯作者：
Freshney, RI
中科院分区：
文献类型：
--
作者：
Freshney, RI
无论是在学术研究还是在工业生物技术中，培养的细胞系都已经成为一种极其宝贵的资源。然而，它们的价值经常受到错误识别和未检测到的微生物污染的影响。正如本卷其他部分所详细描述的那样，这项技术，既简单又复杂，只要有应用的意愿，就可以补救误认和污染的问题。结合对细胞系起源和历史的适当记录，鉴定和污染检测有助于细胞系的起源。详细的记录应从细胞系的启动或接收开始，并应包含捐赠者的数据以及细胞系的来源组织，应继续维护细节，并包括任何意外和故意偏离正常维护的情况。记录还应包含认证的细节和对污染的定期检查。有了这些信息，最好存储在数据库中，并进行适当的备份，这样创建的细胞系的来源使该细胞系成为更有价值的资源，适合于工业应用中的验证，并且当传播用于其他实验室的研究时，更有可能提供可重现的实验结果。
Cultured cell lines have become an extremely valuable resource, both in academic research and in industrial biotechnology. However, their value is frequently compromised by misidentification and undetected microbial contamination. As detailed elsewhere in this volume, the technology, both simple and sophisticated, is available to remedy the problems of misidentification and contamination, given the will to apply it. Combined with proper records of the origin and history of the cell line, assays for authentication and contamination contribute to the provenance of the cell line. Detailed records should start from the initiation or receipt of the cell line, and should incorporate data on the donor as well as the tissue from which the cell line was derived, should continue with details of maintenance, and include any accidental as well as deliberate deviations from normal maintenance. Records should also contain details of authentication and regular checks for contamination. With this information, preferably stored in a database, and suitable backed up, the provenance of the cell line so created makes the cell line a much more valuable resource, fit for validation in industrial applications and more likely to provide reproducible experimental results when disseminated for research in other laboratories.