A CONSTANT RADIUS OF CURVATURE MODEL FOR THE ORGANIZATION OF DNA IN TOROIDAL CONDENSATES
A CONSTANT RADIUS OF CURVATURE MODEL FOR THE ORGANIZATION OF DNA IN TOROIDAL CONDENSATES
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DOI：
10.1073/pnas.92.8.3581
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发表时间：
1995-04-11
影响因子：
11.1
通讯作者：
BALHORN, R
中科院分区：
文献类型：
--
作者：
HUD, NV;DOWNING, KH;BALHORN, R
环状DNA凝聚体由于可能与DNA在病毒中的包装以及作为有序DNA凝聚的模型而受到相当大的关注。环状DNA组织主要支持线轴状模型。然而，我们的观察表明，实际的组织可能会有很大不同。我们提出了另一种模型，在该模型中，给定环面的DNA被组织在一系列大小相等的连续环中，这些环围绕环面轴前进。文中还给出了环形成过程的相关模型。这个动力学模型预测了从溶液中凝聚的DNA的环状尺寸分布，与实验数据符合得很好。
Toroidal DNA condensates have received considerable attention for their possible relationship to the packaging of DNA in viruses and in general as a model of ordered DNA condensation. A spool-like model has primarily been supported for DNA organization within toroids. However, our observations suggest that the actual organization may be considerably different. We present an alternate model in which DNA for a given toroid is organized within a series of equally sized contiguous loops that precess about the toroid axis. A related model for the toroid formation process is also presented. This kinetic model predicts a distribution of toroid sizes for DNA condensed from solution that is in good agreement with experimental data.