A Comparative Chromosome Mapping Study in Japanese Podismini Grasshoppers (Orthoptera: Acrididae: Melanoplinae)
A Comparative Chromosome Mapping Study in Japanese Podismini Grasshoppers (Orthoptera: Acrididae: Melanoplinae)
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DOI：
10.1159/000487063
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发表时间：
2018-01-01
影响因子：
1.7
通讯作者：
Warchalowska-Sliwa, Elzbieta
中科院分区：
文献类型：
--
作者：
Grzywacz, Beata;Tatsuta, Haruki;Warchalowska-Sliwa, Elzbieta
本文报道了日本短足蛛属7个种的核型，即贝氏短足蛛Anapodisma beybienkoi，冲绳短足蛛Fruhstorferiola okinawaensis，日本短足蛛Parapodisma caelestis，日本短足蛛P. mikado，日本短足蛛P. setouchiensis，日本短足蛛P. tenryuensis和斑点短足蛛Sinopodisma punctata（2n雄性= 21，均为近端着丝粒染色体），在常规染色体组的基础上进行了描述和比较（C显带、DAPI/CMA（3）-染色、Ag-NOR）和分子遗传学染色（18 SrDNA和端粒探针的FISH）。这是首次报道A. beybienkoi和P. caelestis。差异染色技术显示了这些物种的核型多样性。18 SrDNA信号的数目为2 ~ 6个，信号位于常染色体或性染色体上。在所有物种中，rDNA簇与Ag-NORs一致。端粒信号在粗线期出现在染色体末端，在减数分裂的其他阶段很少出现。在Anapodisma，Fruhstorferiola和Parapodisma物种的染色体中检测到了组成性异染色质的近着丝粒和一些远端和间质块。用DAPI和CMA（3）染色显示2组异染色质组成。此外，种内的rDNA簇和C-带的数目的差异，观察内的Parapodisma种。基于细胞遗传学特征的证据，不能支持Tonkinacridina的单系性。(C)2018 S.Karger AG，巴塞尔
In the present paper, karyotypes of 7 Japanese Podismini species, Anapodisma beybienkoi, Fruhstorferiola okinawaensis, Parapodisma caelestis, P. mikado, P. setouchiensis, P. tenryuensis, and Sinopodisma punctata (2n male = 21, all acrocentric), are described and compared on the basis of conventional (Cbanding, DAPI/CMA(3) -staining, Ag-NOR) and molecular (FISH with 18S rDNA and telomeric probes) cytogenetic staining methods. This is the first study to report karyotypes of A. beybienkoi and P. caelestis. Differential staining techniques showed karyotypic diversity in these species. The number of 18S rDNA signals ranged from 2 to 6, and the signals were located on the autosomes or sex chromosomes. In all species, clusters of rDNA coincided with Ag-NORs. Telomeric signals occurred at the chromosome ends at the pachytene stage and seldom at other stages of meiosis. Paracentromeric and some distal and interstitial blocks of constitutive heterochromatin were detected in the chromosomes of Anapodisma, Fruhstorferiola, and Parapodisma species. Staining with DAPI and CMA(3) revealed 2 groups of heterochromatin composition. In addition, intraspecific differences in the number of rDNA clusters and C-bands were observed within Parapodisma species. Based on the evidence of cytogenetic characteristics, the monophyly of Tonkinacridina cannot be supported. (C) 2018 S.Karger AG, Basel