A Drosophila protein related to the human zinc finger transcription factor PRDII/MBPI/HIV-EP1 is required for dpp signaling.
A Drosophila protein related to the human zinc finger transcription factor PRDII/MBPI/HIV-EP1 is required for dpp signaling.
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发表时间：
1995-10
期刊：
影响因子：
4.6
通讯作者：
K. Staehling‐Hampton;A. Laughon;F. Hoffmann
中科院分区：
文献类型：
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作者：
K. Staehling‐Hampton;A. Laughon;F. Hoffmann
细胞对哺乳动物TGF-β或果蝇TGF-β相关因子十肢麻痹（dpp）的信号转导途径知之甚少。在这里，我们描述了果蝇schnurri（shn），一个假定的参与DPP信号的转录因子的遗传和分子特征。shn蛋白有8个锌指，与人类转录因子PRDII/MBPI/HIV-EP 1相关，其结合核因子κ B结合位点并激活HIV长末端重复序列（LTR）的转录。SHN mRNA在胚胎中以动态模式表达，所述胚胎包括大多数已知的DPP靶组织，包括背胚盘、中胚层和中肠的副分段4和7。shn的突变影响了几个由dpp调控的发育过程，包括内脏中胚层细胞命运的诱导，外侧外胚层的背/腹模式和翅静脉的形成。shn功能的缺失阻断了由异位dpp表达引起的胚胎中胚层同源结构域蛋白风笛的扩增表达，说明dpp作用下游需要shn功能。我们的结论是，shn功能是至关重要的细胞对dpp的适当反应，并提出shn蛋白是第一个确定的下游组成部分的信号转导通路所使用的dpp及其受体。
Little is known about the signal transduction pathways by which cells respond to mammalian TGF-beta s or to decapentaplegic (dpp), a Drosophila TGF-beta-related factor. Here we describe the genetic and molecular characterization of Drosophila schnurri (shn), a putative transcription factor implicated in dpp signaling. The shn protein has eight zinc fingers and is related to a human transcription factor, PRDII/MBPI/HIV-EP1, that binds to nuclear factor-kappa B-binding sites and activates transcription from the HIV long terminal repeat (LTR). shn mRNA is expressed in a dynamic pattern in the embryo that includes most of the known target tissues of dpp, including the dorsal blastoderm, the mesodermal germlayer and parasegments 4 and 7 of the midgut. Mutations in shn affect several developmental processes regulated by dpp including induction of visceral mesoderm cell fate, dorsal/ventral patterning of the lateral ectoderm and wing vein formation. Absence of shn function blocks the expanded expression of the homeodomain protein bagpipe in the embryonic mesoderm caused by ectopic dpp expression, illustrating a requirement for shn function downstream of dpp action. We conclude that shn function is critical for cells to respond properly to dpp and propose that shn protein is the first identified downstream component of the signal transduction pathway used by dpp and its receptors.