Helical reconstruction in RELION
Helical reconstruction in RELION
复制标题
DOI：
10.1101/095034
复制
发表时间：
2016-12
影响因子：
3
通讯作者：
Shaoda He;S. Scheres
中科院分区：
文献类型：
--
作者：
Shaoda He;S. Scheres
我们描述了一个新的实施重建的螺旋装配的经验贝叶斯框架的RELION。我们的方法通过在傅立叶空间中嵌入螺旋对称算子来计算3D重建的最佳线性滤波器，并通过高斯形先验来处理各个片段方向上的完美螺旋对称偏差。通过将我们的方法整合到RELION中的单粒子分析标准管道中，我们的实现旨在让非经验丰富的用户轻松访问。虽然我们的实现并没有解决的问题，严重不正确的结构时，可以得到错误的螺旋对称性，我们表明四个不同的测试情况下，它是能够重建结构，近原子的分辨率。缩写CARD胱天蛋白酶激活和募集结构域EM电子显微术FSC傅立叶壳层相关IHRSR迭代螺旋实空间重建MAVS线粒体抗病毒信号蛋白TMV烟草花叶病毒
We describe a new implementation for the reconstruction of helical assemblies in the empirical Bayesian framework of RELION. Our approach calculates optimal linear filters for the 3D reconstruction by embedding helical symmetry operators in Fourier-space, and deals with deviations from perfect helical symmetry through Gaussian-shaped priors on the orientations of individual segments. By incorporating our approach into the standard pipeline for single-particle analysis in RELION, our implementation aims to be easily accessible for non-experienced users. Although our implementation does not solve the problem that grossly incorrect structures can be obtained when the wrong helical symmetry is imposed, we show for four different test cases that it is capable of reconstructing structures to near-atomic resolution. Abbreviations CARD Caspase Activation and Recruitment Domain EM Electron Microscopy FSC Fourier Shell Correlation IHRSR Iterative Helical Real-Space Reconstruction MAVS Mitochondrial Antiviral Signaling Protein TMV Tobacco Mosaic Virus