IDENTIFICATION OF CENTROSOMAL PROTEINS IN A HUMAN LYMPHOBLASTIC CELL-LINE
IDENTIFICATION OF CENTROSOMAL PROTEINS IN A HUMAN LYMPHOBLASTIC CELL-LINE
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DOI：
10.1002/j.1460-2075.1986.tb04533.x
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发表时间：
1986-10-01
期刊：
影响因子：
11.4
通讯作者：
BORNENS, M
中科院分区：
文献类型：
--
作者：
GOSTITESTU, F;MARTY, MC;BORNENS, M
分析了来自人T淋巴母细胞KE 37的中心体的高度富集制剂的蛋白质含量。多肽的特定模式的特点是丰富的高分子量的子集。野生型蛋白质和一个主要的蛋白质组与摩尔。wt范围为50至65 kD。几个免疫反应蛋白质进行了鉴定，使用兔血清自发与人类中心体反应。他们包括一个家庭的高摩尔。wt范围为180 - 250 kd，130-kd蛋白和60-65 kd双联体。这些抗原具有以下特性：（1）它们位于中心粒周围物质中;（2）它们的丰度，如中心体标记所判断的，在细胞周期中显著变化，在间相纺锤体的极点处观察到最大值;（iii）在中心体不再充当成核中心的紫杉醇处理的细胞中，它们在有丝分裂细胞和间期细胞中微管阵列的一端重新分布，正如对成核或加帽蛋白质所预期的那样。所有这些性质与它们参与微管成核是相容的。
Highly enriched preparations of centrosomes from human T-lymphoblasts KE 37 were analyzed for their protein content. The specific pattern of polypeptides was characterized by an abundant subset of high mol. wt proteins and a major group of proteins with mol. wt ranging from 50 to 65 kd. Several immunoreactive proteins were identified, using a rabbit serum spontaneously reacting with human centrosomes. They include a family of high mol. wt ranging from 180 to 250 kd, a 130-kd protein and a 60-65 kd doublet. These antigens have the following properties: (1) they are localized within the pericentriolar material; (ii) their abundance, as judged by centrosome labelling, changes significantly during the cell cycle, the maximum being observed at the pole of the metaphasic spindle; (iii) in Taxol-treated cells where the centrosome is no longer acting as a nucleating center, they redistribute at one end of the microtubule arrays in both mitotic and interphasic cells, as expected for nucleating, or capping, proteins. All these properties are compatible with their involvement in microtubule nucleation.