Development and fertility of ovaries in the B6.YDOM sex-reversed female mouse.
Development and fertility of ovaries in the B6.YDOM sex-reversed female mouse.
复制标题
B6.YDOM 性别逆转雌性小鼠卵巢的发育和生育能力。
作者：
T. Taketo;Y. Nishioka;C. Nagamine;I. Villalpando;H. Merchant
将小家鼠Y染色体导入C57 BL/6（B6）小鼠，其XY子代（B6.YDOM）中有一半发育出双侧卵巢和雌性内外生殖器。我们检查了B6.YDOM性别逆转雌性小鼠的生育力。通过与小鼠Y染色体特异性DNA探针的斑点杂交来鉴定个体小鼠的染色体性别。结果表明，所有XY女性缺乏规则的发情周期，但大多数能够交配和排卵后，促性腺激素治疗。当它们被切除卵巢并移植XX雌性同窝配偶的卵巢时，它们开始发情周期。相反，接受XY卵巢移植的XX雌性动物没有恢复发情周期。XY卵巢的发育在形态学上与XX卵巢相当，直至妊娠16天（d.g.），此时大多数生殖细胞已进入减数分裂前期的偶线期或粗线期。然而，在分娩当天（19或20日），XY卵巢髓索中无卵母细胞残留。在对照XX卵巢中，第一代卵泡在髓质区发育，并且5 δ-3 β-羟基类固醇脱氢酶（3 β-HSDH）活性在出生后10天首先出现在生长卵泡周围的基质细胞中。相反，在XY卵巢中，卵泡没有形成在髓质区，3 β-HSDH活性出现在无卵母细胞的髓质索的上皮细胞中。XX和XY卵巢皮质区的原始卵泡继续发育。这些结果表明，XY女性不孕是由于XY卵巢内的缺陷。卵母细胞在髓索中的产前丢失可能是导致内分泌功能异常的关键事件，从而导致发情周期的缺失。
When the Y chromosome of Mus musculus domesticus (YDOM) was introduced onto the C57BL/6 (B6) mouse background, half of the XY progeny (B6.YDOM) developed bilateral ovaries and female internal and external genitalia. We examined the fertility of the B6.YDOM sex-reversed female mouse. The chromosomal sex of the individual mouse was identified by dot hybridization with mouse Y chromosome-specific DNA probes. The results indicated that all XY females lacked regular estrous cyclicity although most were able to mate and ovulate after treatment with gonadotropins. When they had been ovariectomized and grafted with ovaries from the XX female litter mate, they initiated estrous cyclicity. Reciprocally, the XX female that had received XY ovarian grafts did not resume estrous cyclicity. Development of the XY ovary was morphologically comparable to the XX ovary until 16 day of gestation (d.g.), when most germ cells had reached the zygotene or pachytene stage of meiotic prophase. However, by the day of delivery (19 or 20 d.g.), no oocyte remained in the medullary cords of the XY ovary. In the control XX ovary, the first generation of follicles developed in the medullary region, and 5 delta-3 beta-hydroxysteroid dehydrogenase (3 beta-HSDH) activity appeared first in the stromal cells around growing follicles by 10 days after birth. In contrast, in the XY ovary, follicles were not formed in the medullary region, and 3 beta-HSDH activity appeared in epithelial cells of the oocyte-free medullary cords. Primordial follicles in the cortex region continued development in both the XX and XY ovaries. These results suggest that the XY female is infertile due to a defect inside the XY ovary. The prenatal loss of oocytes in the medullary cords may be a key event leading to abnormal endocrine function, and thereby, the absence of estrous cyclicity.
影响因子：
3.6
作者：
NELSON, JF;FELICIO, LS;FINCH, CE
通讯作者：
FINCH, CE