Kinetics of methyl t-butyl ether cometabolism at low concentrations by pure cultures of butane-degrading bacteria
Kinetics of methyl t-butyl ether cometabolism at low concentrations by pure cultures of butane-degrading bacteria
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DOI：
10.1128/aem.67.5.2197-2201.2001
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发表时间：
2001-05-01
影响因子：
4.4
通讯作者：
Georgiou, G
中科院分区：
文献类型：
--
作者：
Liu, CY;Speitel, GE;Georgiou, G
丁烷氧化节杆菌（ATCC 27778）细菌被证明可以降解低浓度的甲基t-丁基醚（MTBE，范围为100至800杯/升），其表观半饱和浓度（k -s）为2.14 mg/升，最大底物利用率（k(c)）为0.43 mg/mg每天的总悬浮固体。节杆菌细菌在丁烷上生长时表现出MTBE降解活性，而在葡萄糖、丁醇或tryptose phosphate肉汤上生长时则没有。丁烷、叔丁醇或乙炔的存在对MTBE的降解率有负面影响。三磷酸甲氧霉OB3b和灰色链霉菌都不能代谢MTBE。
Butane-oxidizing Arthrobacter (ATCC 27778) bacteria were shown to degrade low concentrations of methyl t-butyl ether (MTBE; range, 100 to 800 mug/liter) with an apparent half-saturation concentration (K-s) of 2.14 mg/liter and a maximum substrate utilization rate (k(c)) of 0.43 mg/mg of total suspended solids per day, Arthrobacter bacteria demonstrated MTBE degradation activity when grown on butane but not when grown on glucose, butanol, or tryptose phosphate broth. The presence of butane, tert-butyl alcohol, or acetylene had a negative impact on the MTBE degradation rate. Neither Methylosinus trichosporium OB3b nor Streptomyces griseus was able to cometabolize MTBE.