Pyricularia grisea Causing Gray Leaf Spot of Perennial Ryegrass Turf: Population Structure and Host Specificity.
Pyricularia grisea Causing Gray Leaf Spot of Perennial Ryegrass Turf: Population Structure and Host Specificity.
复制标题
引起多年生黑麦草草坪灰斑病的稻瘟病菌：种群结构和寄主特异性。
DOI：
10.1094/pdis.2001.85.8.817
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发表时间：
2001
期刊：
影响因子：
4.5
通讯作者：
D. Huff
中科院分区：
文献类型：
--
作者：
G. Viji;B. Wu;S. Kang;W. Uddin;D. Huff
灰斑病是美国多年生黑麦草（Lolium perenne）草坪的一种严重疾病。使用分子标记和对各种禾本科宿主的致病性测定来表征多年生黑麦草中引起该疾病的稻瘟病菌分离株。使用不同类型的转座子作为探针来确定多年生黑麦草分离株之间的遗传关系。使用 Pot2 和 MGR586 探针进行的系统发育分析，并使用 AMOVA（分子方差分析）进行分析，表明这些来自多年生黑麦草的分离株由三个密切相关的谱系组成。所有分离株均属于单一交配型 MAT1-2。在来自除多年生黑麦草以外的 16 个寄主物种的 20 个分离株中，只有小麦 (Triticum aestivum) 和小黑麦 (× Triticosecale) 的分离株与多年生黑麦草分离株的 Pot2 指纹显示出显着的相似性。来自多年生黑麦草的 P. grisea 分离株中 Pot2 和 MGR586 的拷贝数和指纹表明，它们在遗传上与源自美国水稻 (Oryza sativa) 的分离株不同。多年生黑麦草分离株在编码核糖体RNA的基因的内部转录间隔区(ITS)区域也具有与小麦和小黑麦分离株相同的序列，并表现出水稻分离株的序列多态性。在致病性测定中，来自 Legacy II 多年生黑麦草的所有稻瘟病菌菌株均对 Marilee 软白冬小麦、Bennett 硬红冬小麦、Era 软白春小麦和 Presto 小黑麦造成了特征性的稻瘟病症状，并且对这些寄主具有高毒力。来自小麦的分离物和来自小黑麦（来自巴西）的分离物对多年生黑麦草和 Rebel III 高羊茅（Festuca arundinacea）也具有高毒力。多年生黑麦草中的任何分离株都不会引起拉格鲁稻的疾病，反之亦然。了解感染多年生黑麦草的稻瘟病菌分离株的种群结构及其与其他禾本科宿主分离株的相关性可能有助于识别该病原体的替代宿主。
Gray leaf spot is a serious disease of perennial ryegrass (Lolium perenne) turf in the United States. Isolates of Pyricularia grisea causing the disease in perennial ryegrass were characterized using molecular markers and pathogenicity assays on various gramineous hosts. Genetic relationships among perennial ryegrass isolates were determined using different types of trans-posons as probes. Phylogenetic analysis using Pot2 and MGR586 probes, analyzed with AMOVA (analysis of molecular variance), showed that these isolates from perennial ryegrass consist of three closely related lineages. All the isolates belonged to a single mating type, MAT1-2. Among 20 isolates from 16 host species other than perennial ryegrass, only the isolates from wheat (Triticum aestivum) and triticale (× Triticosecale), showed notable similarity to the perennial ryegrass isolates based on their Pot2 fingerprints. The copy number and fingerprints of Pot2 and MGR586 in isolates of P. grisea from perennial ryegrass indicate that they are genetically distinct from the isolates derived from rice (Oryza sativa) in the United States. The perennial ryegrass isolates also had the same sequence in the internal transcribed spacer (ITS) region of the genes encoding ribosomal RNA as that of the wheat and triticale isolates, and exhibited rice isolate sequence polymorphisms. In pathogenicity assays, all the isolates of P. grisea from Legacy II perennial ryegrass caused characteristic blast symptoms on Marilee soft white winter wheat, Bennett hard red winter wheat, Era soft white spring wheat, and Presto triticale, and they were highly virulent on these hosts. An isolate from wheat and one from triticale (from Brazil) were also highly virulent on perennial ryegrass and Rebel III tall fescue (Festuca arundinacea). None of the isolates from perennial ryegrass caused the disease on Lagrue rice, and vice versa. Understanding the population structure of P. grisea isolates infecting perennial ryegrass and their relatedness to isolates from other gramineous hosts may aid in identifying alternate hosts for this pathogen.