AMINO-ACID-SEQUENCE OF THE PLASMA-MEMBRANE ATPASE OF NEUROSPORA-CRASSA - DEDUCTION FROM GENOMIC AND CDNA SEQUENCES
AMINO-ACID-SEQUENCE OF THE PLASMA-MEMBRANE ATPASE OF NEUROSPORA-CRASSA - DEDUCTION FROM GENOMIC AND CDNA SEQUENCES
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DOI：
10.1073/pnas.83.20.7693
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发表时间：
1986-10-01
影响因子：
11.1
通讯作者：
SLAYMAN, CW
中科院分区：
文献类型：
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作者：
HAGER, KM;MANDALA, SM;SLAYMAN, CW
粗糙脉孢菌的质膜含有产电H+-ATP酶（EC 3.6.1.35），我们已经分离并测序了其基因组和cDNA克隆。ATP酶基因被四个小内含子（58-124个碱基对）中断。它编码920个氨基酸（Mr，99，886）的蛋白质，具有多达8个跨膜片段。链孢菌ATP酶与动物细胞的Na+、K+和Ca 2+转运ATP酶显示出显著的氨基酸序列同源性，特别是在似乎参与ATP结合和水解的区域。
The plasma membrane of Neurospora crassa contains as electrogenic H+-ATPase (EC 3.6.1.35) for which we have isolated and sequenced both genomic and cDNA clones. The ATPase gene is interrupted by four small introns (58-124 base pairs). It encodes a protein of 920 amino acids (Mr, 99,886) possessing as many as eight transmembrane segments. The Neurospora ATPase shows significant amino acid sequence homology with the Na+,K+- and Ca2+-transporting ATPases of animal cells, particuarly in regions that appear to be involved in ATP binding and hydrolysis.