DYNAMICS, STRUCTURE, AND FUNCTION ARE COUPLED IN THE MITOCHONDRIAL MATRIX
DYNAMICS, STRUCTURE, AND FUNCTION ARE COUPLED IN THE MITOCHONDRIAL MATRIX
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DOI：
10.1073/pnas.88.18.8057
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发表时间：
1991-09-01
影响因子：
11.1
通讯作者：
HACKENBROCK, CR
中科院分区：
文献类型：
--
作者：
SCALETTAR, BA;ABNEY, JR;HACKENBROCK, CR
利用荧光各向异性技术和电子显微镜研究了大鼠肝线粒体基质的结构和功能与分子扩散之间的耦合。结果证实，基质超微结构和基质蛋白浓度受线粒体呼吸状态和外部介质渗透压的影响。在生理渗透压，荧光代谢物大小的探针被发现在线粒体基质中缓慢扩散，但不是完全不动。此外，扩散速率的显着差异被发现存在于不同的线粒体呼吸状态之间，最慢的扩散发生在具有最高基质蛋白浓度的状态。这些数据支持的概念，扩散是相当大的阻碍，由于有限的探针可访问的水的基质结构，并进一步表明，体积依赖性调节基质蛋白包装可能会调节代谢物的扩散，进而，线粒体代谢。
The coupling between molecular diffusion and the structure and function of the rat liver mitochondrial matrix was explored using fluorescence anisotropy techniques and electron microscopy. The results confirm that matrix ultrastructure and the concentration of matrix protein are influenced by the respiratory state of mitochondria and the osmolarity of the external medium. At physiological osmolarity, a fluorescent metabolite-sized probe was found to diffuse slowly in the mitochondrial matrix but not to be completely immobile. In addition, significant differences in diffusion rates were found to exist between different mitochondrial respiratory states, with the slowest diffusion occurring in states with the highest matrix protein concentration. These data support the concept of a matrix structure in which diffusion is considerably hindered due to limited probe-accessible water and further suggest that volume-dependent regulation of matrix protein packing may modulate metabolite diffusion and, in turn, mitochondrial metabolism.