A role for thyroid hormone in wound healing through keratin gene expression
A role for thyroid hormone in wound healing through keratin gene expression
复制标题
DOI：
10.1210/en.2003-1696
复制
发表时间：
2004-05-01
期刊：
影响因子：
4.8
通讯作者：
Holick, MF
中科院分区：
文献类型：
--
作者：
Safer, JD;Crawford, TM;Holick, MF
甲状腺激素(TH)在伤口愈合中的重要性尚不清楚。为了获得洞察力，我们评估了TH缺乏对培养的角质形成细胞中伤口愈合基因的影响。RT-PCR法显示，角蛋白6a(K6a)和角蛋白16(K16)基因在TH缺失细胞中的表达分别是TH缺失细胞的3.8%(P&0.005)和1.90倍(P<；0.05)。实时定量聚合酶链式反应显示，K6a、K16和K17的完整细胞表达水平分别为18-(P<；0.001)、10-(P<；0.001)和4倍(P<；0.005)。为了检验对最佳伤口愈合的要求，我们对比了TH缺陷小鼠和IP T-3处理的小鼠。伤后4天，ip T-3治疗的小鼠伤口闭合程度是甲状腺功能减退小鼠的两倍(P<；0.001)。反转录-聚合酶链式反应显示，K6a和K17基因在正常小鼠皮肤中的表达分别是甲减小鼠皮肤的5倍(P&0.001)和1.7倍(P<；0.05)。T-3对于角质形成细胞的增殖是必需的，而角质形成细胞是最佳伤口愈合所必需的。T-3通过刺激伤口修复角蛋白基因的表达而发挥影响。因此，对于正在接受手术的甲状腺功能减退患者，我们的数据支持围手术期的T-3治疗。
The importance of thyroid hormone (TH) in wound healing is not well understood. To gain insight, we evaluated the impact of TH deficiency on wound-healing genes in cultured keratinocytes. By RT-PCR, keratin 6a (K6a) and 16 (K16) gene expression in TH replete cells was 3.8- (P < 0.005) and 1.9-fold (P < 0.05) greater, respectively, than expression in TH-deficient cells. By real-time PCR, TH replete cell expression of K6a, K16, and K17 was greater than in deficient cells: 18- ( P < 0.001), 10- ( P < 0.001), and 4-fold ( P < 0.005), respectively. To examine TH requirement for optimal wound healing, we contrasted TH-deficient vs. ip T-3-treated mice. Four days after wounding, ip T-3-treated mice had twice the degree of wound closure as hypothyroid mice ( P < 0.001). By RT-PCR, K6a and K17 gene expression from control mouse skin was greater than from hypothyroid mouse skin: 5- ( P < 0.001) and 1.7- fold ( P < 0.05), respectively. T-3 is necessary for the keratinocyte proliferation required for optimal wound healing. T-3 exerts influence by stimulating expression of the wound-healing keratin genes. Thus, for hypothyroid patients undergoing surgery that cannot be delayed until euthyroidism is achieved, our data support T-3 treatment for the perioperative period.