Angiogenic potential of CD44+ CD90+ multipotent CNS stem cells in vitro
Angiogenic potential of CD44+ CD90+ multipotent CNS stem cells in vitro
复制标题
DOI：
10.1016/j.bbrc.2006.08.135
复制
发表时间：
2006-10-27
影响因子：
3.1
通讯作者：
Ito-Dufros, Yuko
中科院分区：
文献类型：
--
作者：
Oishi, Kazuhiko;Ito-Dufros, Yuko
尽管现在已经清楚神经干细胞（NSC）的几个亚群存在于早期发育和成年期，但NSC的血管生成潜力仍然是一个争论的主题。在此，我们报道了从原代神经球中分离的CD 44（+）CD 90（+）细胞可以在体外形成血管管结构。从小鼠胚胎皮层分离的神经干细胞在含有20 ng/ml碱性成纤维细胞生长因子（bFGF）的无血清培养基中培养时形成神经球。使用EPICS ALTRA流式细胞仪和抗CD 44和CD 90的抗体从神经球中富集CD 44（+）CD 90（+）细胞。纯化后的CD 44（+）CD 90（+）细胞形成神经球，并分化为神经元和星形胶质细胞。将细胞接种于胶原凝胶中，加入20%胎牛血清和bFGF培养7天，可形成血管管状结构。这些结果表明，CD 44（+）CD 90（+）细胞具有生成神经球和形成血管管的能力。(c)2006年爱思唯尔公司All rights reserved.
Although it is now clear that several subpopulations of neural stem cells (NSCs) exist during early development and adulthood, the angiogenic potential of NSCs remains a subject of debate. Here, we report that CD44(+) CD90(+) cells isolated from primary neurospheres can form vascular-tube structures in vitro. NSCs isolated from the mouse embryonic cortex formed neurospheres when cultured in serum-free medium containing 20 ng/ml basic fibroblast growth factor (bFGF). CD44(+) CD90(+) cells were enriched from the neurospheres using an EPICS ALTRA flow cytometer, and antibodies against CD44 and CD90. The purified CD44(+) CD90(+) cells generated neurospheres, and differentiated into neurons and astrocytes. When the cells were inoculated into collagen gels and cultured with 20% fetal bovine serum plus bFGF for 7 days, vascular tube-like structures were formed. These results indicate that CD44(+) CD90(+) cells have the ability to generate neurospheres and to form vascular tubes. (c) 2006 Elsevier Inc. All rights reserved.