Bio-Inspired Degradable Polyethylenimine/Calcium Phosphate Micro-/Nano-Composites for Transient Ultrasound and Photoluminescence Imaging
Bio-Inspired Degradable Polyethylenimine/Calcium Phosphate Micro-/Nano-Composites for Transient Ultrasound and Photoluminescence Imaging
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DOI：
10.1021/acs.chemmater.2c00857
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发表时间：
2022-08
影响因子：
8.6
通讯作者：
Tengyu He;David G. Bradley;Ming Xu;Shu-Ting Ko;Baiyan Qi;Yi Li;Yong Cheng;Zhicheng Jin
中科院分区：
文献类型：
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作者：
Tengyu He;David G. Bradley;Ming Xu;Shu-Ting Ko;Baiyan Qi;Yi Li;Yong Cheng;Zhicheng Jin
无机纳米材料在治疗诊断学中具有巨大的潜力，但其转化受到不可降解性产生的毒性的限制。块状磷酸盐玻璃具有良好的生物降解性、生物相容性，并且易于掺入成像染料和药物。然而，这种材料的微米/纳米粒子的简便且温和的基于溶液的合成仍有待探索。受硅藻生物硅化过程的启发，我们通过胺（PEI）和羟基（磷酸盐）之间的氢键创建了聚乙烯亚胺（PEI）/磷酸盐聚集体，并通过动态光散射和1H溶液核磁共振得到证明。钙前体和 PEI/磷酸盐聚集体之间的溶胶-凝胶反应产生了可降解的 PEI/磷酸钙 (PEI/CP) 微/纳米复合材料，具有多种尺寸（396 ± 128 nm 至 63 ± 8 μm）和形态（六角形微盘、微花、微叶、纳米蝴蝶和 纳米带）。检查了 PEI/CP 的组成和化学结构。 PEI/CP 的细胞毒性可忽略不计，并会在 24 小时内降解。体外研究显示了 PEI/CP 在瞬态超声和光致发光成像中的应用前景。
Inorganic nanomaterials hold immense potential in theranostics, but their translation is limited by the toxicity resulting from non-degradability. Bulk phosphate-based glasses offer great biodegradability, biocompatibility, and ease in incorporating imaging dyes and drugs. However, the facile and mild solution-based synthesis of micro-/nanoparticles of this material is yet to be explored. Inspired by the biosilicification process in the diatom, we created polyethylenimine (PEI)/phosphate aggregates via the hydrogen bonding between amine (PEI) and hydroxyl (phosphate) groups, which was proved by dynamic light scattering and1H solution nuclear magnetic resonance. The sol–gel reaction between the calcium precursor and PEI/phosphate aggregates yielded degradable PEI/calcium phosphate (PEI/CP) micro-/nano-composites with versatile sizes (396 ± 128 nm to 63 ± 8 μm) and morphologies (hexagonal micro-disc, micro-flower, micro-leaf, nano-butterfly, and nano-ribbon). The PEI/CP composition and chemical structure were examined. PEI/CP has negligible cell cytotoxicity and degrades within 24 h. In vitro studies showed the promise for PEI/CP in transient ultrasound and photoluminescence imaging.