Selective calcification of rat brain lesions caused by systemic administration of kainic acid
Selective calcification of rat brain lesions caused by systemic administration of kainic acid
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DOI：
10.14670/hh-18.855
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发表时间：
2003-07-01
影响因子：
2
通讯作者：
Díaz-Flores, L
中科院分区：
文献类型：
--
作者：
Gayoso, MJ;Al-Majdalawi, A;Díaz-Flores, L
神经组织先前受损区域的营养不良性钙化发生在广泛的人类疾病中。在大鼠中研究了星形胶质细胞和小胶质细胞反应与钙盐沉积之间的关系，研究时间长达5个月，大鼠的脑损伤由红藻氨酸的全身给药产生。研究了钙盐沉积物的形貌和原子组成。观察到两种类型的病变，硬化性和增生性。在硬化病变中，星形胶质细胞的增生和肥大部分取代了失去的神经元，在治疗后约25天达到最大值。在增生性病变中，星形细胞反应仅发生在增生区周围。小胶质细胞反应在两种类型的病变中相似，并且在约25天内达到最高表达。钙沉积在骨组织中观察到，但在骨坏死性病变中未观察到。在注射后仅5天，在扫描电子显微镜下观察到在巩膜病变中可清楚区分的钙盐颗粒。钙化颗粒的大小随时间增加，在5个月时直径达到40 μ m或更大。这些存款的原子组成，通过X-射线显微分析研究，表明钙浓度随时间的增加。虽然星形胶质细胞和小胶质细胞反应与钙盐沉积之间没有明确的关系，但全身注射红藻氨酸盐会在骨组织中产生逐渐更大和更集中的钙沉积，但在炎症性病变中不会。
Dystrophic calcification of previously damaged areas of nervous tissue occurs in a wide range of human diseases. The relationship between astroglial and microglial reactions and deposits of calcium salts was studied for up to five months in rats with a brain lesion produced by systemic administration of kainate. The morphology and atomic composition of the calcium salt deposits was also studied. Two types of lesions, sclerotic and liquefactive, were observed. In sclerotic lesions hyperplasia and hypertrophy of astrocytes partially substituted for the lost neurons, reaching a maximum in about twenty-five days after treatment. In liquefactive lesions, the astrocytic reaction occurred only around the liquefactive area. Microglial reaction was similar in both types of lesion and reached its highest expression in about twenty-five days. Calcium deposits were observed in the sclerotic but not in the liquefactive lesions. Clearly distinguishable granules of calcium salts were observed in sclerotic lesions under scanning electron microscopy after only five days post-injection. The size of calcified granules increased with time reaching 40 mum or more in diameter at five months. The atomic composition of these deposits, studied by X-ray microanalysis, showed a time-dependent increase in calcium concentration. While there was no clear relationship between astroglial and microglial reactions and calcium salt deposits, the systemic injection of kainate produced progressively larger and more concentrated calcium deposits in sclerotic, but not in liquefactive lesions.