The use of embryo transfer to produce pregnancies in repeat-breeding dairy cattle
The use of embryo transfer to produce pregnancies in repeat-breeding dairy cattle
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DOI：
10.1016/j.theriogenology.2007.09.001
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发表时间：
2008-01-01
期刊：
影响因子：
2.8
通讯作者：
Koyama, H.
中科院分区：
文献类型：
--
作者：
Dochi, O.;Takahashi, K.;Koyama, H.
重复育种是影响奶牛管理经济成功的重要因素。本研究的目的是调查冻融体外受精胚胎移植在重复繁殖荷斯坦牛妊娠中的有效性。通过从两个当地屠宰场获得的卵巢中抽吸 2-5 毫米的卵泡来收集卵丘卵母细胞复合物。 IVF 后，第 7 天和第 8 天的囊胚冷冻在含 0.1 M 蔗糖的 1.5 M 乙二醇中，或含 0.1 M 蔗糖的 1.4 M 甘油中。荷斯坦受者（122 头小母牛和 410 头奶牛）包括那些在 3-21 次授精后仍未受孕的受者。乙二醇冷冻的胚胎直接移植，甘油冷冻的胚胎在蔗糖中稀释冷冻保护剂后，在发情后7天或8天（无AI组）或AI后（有AI组）移植到受者体内。通过卡方检验比较怀孕率。在小母牛（分别为 49.2% 和 29.5%）和奶牛（分别为 41.5% 和 20.4%；P < 0.05）中，AI 后胚胎移植（有 AI 组）的妊娠率显着高于单独胚胎移植（无 AI 组）。小母牛和奶牛的怀孕率没有显着差异。然而，在使用 AI 组中，妊娠率随着胚胎移植前授精次数的增加而下降，但在未使用 AI 组中则没有。因此，在进行人工授精的同一周期内移植冷冻解冻的 IVF 胚胎可以提高重复配种荷斯坦小母牛和奶牛的妊娠率，并表明胚胎移植是治疗重复配种的替代方案 (c) 2007 Elsevier Inc. 保留所有权利。
Repeat breeding is an important factor affecting economic success in dairy management. The objective of this study was to investigate the effectiveness of transfer of frozen-thawed IVF embryos in establishing pregnancy in repeat-breeding Holstein cattle. Cumulus oocyte complexes were collected by aspiration of 2-5 mm follicles from ovaries obtained at two local abattoirs. After IVF, days 7 and 8 blastocysts were frozen either in 1.5 M ethylene glycol with 0.1 M sucrose, or in 1.4 M glycerol with 0.1 M sucrose. Holstein recipients (122 heifers and 410 cows) included those that had not conceived after 3-21 inseminations. Embryos frozen in ethylene glycol were transferred directly, and embryos frozen in glycerol were transferred after dilution of the cryoprotectant in sucrose into recipients 7 or 8 days after estrus (without-AI group), or following AI (with-AI group). Pregnancy rates were compared by the Chi-square test. Significantly higher pregnancy rates were achieved by embryo transfer following AI (with-AI group) than by embryo transfer alone (without-AI group) in both heifers (49.2 and 29.5%, respectively) and cows (41.5 and 20.4%, respectively; P < 0.05). Pregnancy rates were not significantly different between heifers and cows. However, pregnancy rate decreased as the number of inseminations prior to embryo transfer increased in the with-AI group, but not in the without-AI group. Therefore, transfer of frozen-thawed IVF embryos during the same cycle in which AI was done improved pregnancy rates in repeat-breeding Holstein heifers and cows, and suggested that embryo transfer is an alternative in the treatment of repeat breeding (c) 2007 Elsevier Inc. All rights reserved.