DETERMINATION OF FRUCTANS IN PLANTS: CURRENT ANALYTICAL MEANS FOR EXTRACTION, DETECTION, AND QUANTIFICATION
DETERMINATION OF FRUCTANS IN PLANTS: CURRENT ANALYTICAL MEANS FOR EXTRACTION, DETECTION, AND QUANTIFICATION
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DOI：
10.1002/9781119312994.apr0672
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发表时间：
2019-02-01
影响因子：
1.6
通讯作者：
Burton, Rachel
中科院分区：
文献类型：
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作者：
Matros, Andrea;Peukert, Manuela;Burton, Rachel
果聚糖是高等植物和微生物中最重要的蔗糖衍生水溶性碳水化合物之一。它们的主要区别在于末端蔗糖和相邻果糖基单元之间的连接类型，以及从低聚物 (DP 3-9) 到聚合物 (DP >= 10 到几百) 的聚合度，以及它们是线性结构还是高度支化结构（第 2 节）。迅速增加的科学证据表明，果聚糖具有储备和结构碳水化合物的功能，并且具有抗氧化和免疫调节/信号潜力，这推动了基础研究以及食品和营养潜在应用的近期发展。相比之下，果聚糖测定和鉴别的分析方法发展缓慢。在本文中，我们总结了有关果聚糖分析方法的当前知识。我们重点关注果聚糖提取和分离（第 3 节）、定量（第 4 节）、分离和检测（第 5 节）、结构表征（第 6 节）以及组织特异性和空间分辨分析方法（第 7 节）以及各种植物材料中果聚糖的无损检测（第 8 节）。最后，我们讨论了植物组织中果聚糖分析的未来前景，也是在研究结构-功能关系的背景下进行的（第 9 节）。
Fructans are one of the most significant groups of sucrose-derived water-soluble carbohydrates in higher plants and microbes. They are largely differentiated by the linkage type between the terminal sucrose and the adjacent fructosyl units as well as by the degree of polymerisation ranging from oligomers (DP 3-9) to polymers (DP >= 10 up to a few hundred) and whether they are linear or highly branched structures (Section 2). The rapidly increasing scientific evidence for fructans functioning as reserve and structural carbohydrates as well as having antioxidant and immunomodulatory/signalling potential has motivated a recent boost in both fundamental research and in potential applications for food and nutrition. In contrast, analytical methods for fructan determination and discrimination have developed slowly. In this article, we summarise the current knowledge concerning methodologies for fructan analysis. We focus on fructan extraction and isolation (Section 3), quantification (Section 4), separation and detection (Section 5), structural characterisation (Section 6) as well as approaches for tissue-specific and spatially resolved analysis (Section 7), and nondestructive detection of fructans (Section 8) in various plant materials. Finally, we discuss future perspectives for fructan analysis in plant tissues, also in the context of investigating structure-function relationships (Section 9).