The application of CRISPR/Cas9 system in the generation of viral vectored avian influenza vaccines
The application of CRISPR/Cas9 system in the generation of viral vectored avian influenza vaccines
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CRISPR/Cas9系统在病毒载体禽流感疫苗生产中的应用
DOI：
10.1099/acmi.ac2019.po0128
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发表时间：
2019
影响因子：
--
通讯作者：
Chang P
中科院分区：
文献类型：
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作者：
Chang P
禽流感是一种高度传染性的家禽疾病，死亡率可达100%，造成巨大的经济负担和对粮食安全的威胁。疫苗接种是预防和控制流感最有效的策略。重组载体疫苗是一种有效的有前途的疫苗，能够免疫多种病原体。构建重组疫苗的传统方法包括同源重组或细菌人工染色体，但这些方法既耗时又费力。聚类规则间隔回文重复序列(CRISPR)/Cas9系统是最近发展起来的一种基因编辑技术，已被证明对基因修饰有益，并为构建重组疫苗提供了一种替代方法。基因插入主要有两种方法；易出错的非同源末端连接（NHEJ）和高保真同源定向修复（HDR）途径。由于其高保真度，大多数研究都将重点放在利用HDR进行疫苗开发上，但NHEJ由于其高效率而具有一些吸引人的优势。在我们的研究中，我们探索了HDR和NHEJ依赖的CRISPR/Cas9系统，以鸭肠炎病毒和火鸡疱疹病毒为载体，快速生成重组流感疫苗。
Avian influenza is highly contagious poultry disease and the mortality can reach 100 %, leading to huge economic burden and threat to food security. Vaccination is the most effective strategy for prevention and control of influenza. Recombinant vector vaccines are effective promising vaccines capable of immunizing against multiple pathogens. Traditional methods for constructing recombinant vaccines involve homologous recombination or bacterial artificial chromosomes but these methods can be time-consuming and labour-intensive. The clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system is a recently developed gene editing technology which has proven beneficial to gene modification and offers an alternative for constructing recombinant vaccines. There are mainly two methods used for gene insertion; error-prone non-homologous end joining (NHEJ) and the high-fidelity homology-directed repair (HDR) pathway. Owing to its high fidelity, most studies focus on using HDR for vaccine development but NHEJ offers some attractive advantages through its high efficiency. In our study, both HDR and NHEJ dependent CRISPR/Cas9 systems were explored for the rapid generation of recombinant influenza vaccines using duck enteritis virus and herpesvirus of turkeys as vectors.