QUANTITATIVE BONE-HISTOLOGY - A NEW METHOD
QUANTITATIVE BONE-HISTOLOGY - A NEW METHOD
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DOI：
10.3109/00313028009060080
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发表时间：
1980-01-01
期刊：
影响因子：
4.5
通讯作者：
EVANS, RA
中科院分区：
文献类型：
--
作者：
DUNSTAN, CR;EVANS, RA
描述了一种使用适合于组织化学的包埋介质对不脱钙骨进行定量组织学的方法。在活检前24天和3天给予正常人受试者（6）四环素标记物，所述活检是使用8号Jamshidi针从髂后棘取得的。将组织包埋在等量甲基丙烯酸甲酯和甲基丙烯酸羟乙酯的混合物中。切下三个连续切片，用组织化学染色酸性磷酸酶活性以鉴定破骨细胞细胞质，用派洛宁染色以鉴定成骨细胞质，固定未染色以进行四环素荧光。使用显微镜的照相机lucida附件叠加3个切片的描记，并使用电子数字化仪定量描记。主要参数为骨面积（17.1 ± 0.001）。4.4%）（平均值。+-。SD）、成骨细胞或形成表面（5.9 . ±. 2.7%）和破骨细胞或再吸收表面（1.0 . ±. 0.3%）。骨沉积率为1.0 . ±-。0.2μ m/天和再吸收速度（通过假设骨形成和再吸收相等来计算）8.4 ±。4.1μ m/天。矿化通过类骨质面积（3.8 ± 0.05）进行评估。类骨质表面（13.9 ±.四环素在矿化前缘的吸收（62.3 ±. 13.5%）和矿化滞后时间（18.1 ±. 2.5天）。
A method is described for quantitative histology of undecalcified bone using an embedding medium suitable for histochemistry. Normal human subjects (6) were given tetracycline markers 24 and 3 days prior to the biopsy which was taken from the posterior iliac spine using an 8 gauge Jamshidi needle. The tissue was embedded in a mixture of equal parts methylmethacrylate and hydroxyethylmethacrylate. Three consecutive sections were cut and stained histochemically for acid phosphatase activity to identify osteoclast cytoplasm, stained with pyronin to identify osteoblast cytoplasm, mounted unstained for tetracycline fluorescence. Tracings of the 3 sections were superimposed using a camera lucida attachment to the microscope, and the tracings quantitated with an electronic digitizer. The major parameters were bone area (17.1 .+-. 4.4%) (mean .+-. SD), osteoblast or forming surface (5.9 .+-. 2.7%) and osteoclast or resorbing surface (1.0 .+-. 0.3%). Bone apposition rate was 1.0 .+-. 0.2 .mu.m/day and resorption velocity (calculated by assuming bone formation and resorption to be equal) 8.4 .+-. 4.1 .mu.m/day. Mineralization was assessed by osteoid area (3.8 .+-. 2.5%), osteoid surface (13.9 .+-. 6.6%), tetracycline uptake at the mineralizing front (62.3 .+-. 13.5%) and mineralization lag time (18.1 .+-. 2.5 days).