GLIAL CELLS METABOLICALLY COOPERATE: A POTENIAL REQUIREMENT FOR GENE REPLACEMENT THERAPY: 102
GLIAL CELLS METABOLICALLY COOPERATE: A POTENIAL REQUIREMENT FOR GENE REPLACEMENT THERAPY: 102
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神经胶质细胞代谢协作：基因替代疗法的潜在要求：102
作者：
R. Koenker;L. A. Luchtman;R. Willis;J. Seegmiller;H. Gruber
放射自显像显示免疫荧光标记的胶质细胞在代谢方面与成纤维细胞合作。对无细胞接触的合作和结合梯度的观察表明，合作是通过放射性标记的腺嘌呤、腺苷或甲基硫代腺苷的细胞外运输发生的。当受体细胞也缺乏腺嘌呤或腺苷回收所需的酶时，这些嘌呤化合物的转移是由合作的定量损失支持的。神经胶质细胞合作的证明为目前对某些先天性代谢错误（如Lesch-Nyhan综合征）的神经系统症状的基因替代疗法的研究提供了动力。
Immunofluorescent-labeled glial cells are shown by radioautography to metabolically cooperate with fibroblasts. The observations of cooperation without cell contact and of incorporation gradients suggest that cooperation occurs through extracellular transport of radiolabeled adenine, adenosine or methylthioadenosine. The transfer of these purine compounds is supported by the quantitative loss of cooperation when the recipient cell is also deficient in enzymes required for adenine or adenosine salvage. The demonstration of glial cell cooperation provides impetus for current research toward gene replacement therapy for the neurologic symptoms of certain inborn errors of metabolism, such as Lesch-Nyhan syndrome.