Ion-pairing reversed-phased chromatography/mass spectrometry of heparin
Ion-pairing reversed-phased chromatography/mass spectrometry of heparin
复制标题
DOI：
10.1016/j.carres.2005.11.030
复制
发表时间：
2006-02-27
影响因子：
3.1
通讯作者：
Ringborg, LH
中科院分区：
文献类型：
--
作者：
Henriksen, J;Roepstorff, P;Ringborg, LH
肝素和肝素衍生成分是广泛应用的抗凝血药，除其他用途外，还用于治疗深静脉血栓和肺栓塞。天然肝素的解聚会导致复杂的硫酸盐线性低聚糖混合物，而这些混合物通常没有得到很好的表征。为了进一步表征这种混合物，开发了两种在线离子对反相色谱电喷雾电离(ESI)质谱学方法。其中一个系统允许测定中分子肝素制剂中的200多个组分，而另一个系统可用于根据大小分离前一次分离后的同分异构体肝素低聚糖。后一个系统允许半制备分离肝素低聚糖的异构体。实验装置包括油线阳离子交换装置，以防止离子配对试剂进入质谱仪。(C)2005爱思唯尔有限公司。保留所有权利。
Heparin and heparin-derived components are widely applied anticoagulant drugs used for amongst other applications medical treatment of deep vein thrombosis and pulmonary embolism. Depolymerisation of native heparin results in complex mixtures of sulfated linear oligosaccharides that are usually not well characterised. In order to further characterise such mixtures, two on-line ion-pairing reverse-phased chromatography electrospray ionisation (ESI) mass spectrometry methods have been developed. One of the systems allows the determination of more than 200 components in a medium molecular weight heparin preparation, whereas the other system can be used to separate isomeric heparin oligosaccharides after previous separation according to size. This latter system allows semi-preparative isolation of isomeric heparin oligosaccharides. The experimental setup includes oil-line cation exchange in order to prevent the ion-pairing reagent from entering the mass spectrometer. (c) 2005 Elsevier Ltd. All rights reserved.