A Multi-model Approach to Assessing Local and Global Cryo-EM Map Quality
A Multi-model Approach to Assessing Local and Global Cryo-EM Map Quality
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DOI：
10.1016/j.str.2018.10.003
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发表时间：
2019-02-05
期刊：
影响因子：
5.7
通讯作者：
Lander, Gabriel C.
中科院分区：
文献类型：
--
作者：
Herzik, Mark A., Jr.;Fraser, James S.;Lander, Gabriel C.
目前还不存在一个标准化的指标，如何以及cryo-EM衍生模型代表密度，他们从产生。我们提出了一个简单的方法，利用免费提供的工具来生成一套独立的模型，并评估其收敛的EM密度。这些分析提供了一个定量和定性的评估模型的精度和他们的代表性的密度，分别，同时提供了一个平台，用于评估全球和本地EM地图的质量。我们进一步使用标准化数据集来提供一套模型内的预期偏差，该模型针对报告为5埃分辨率或更高的EM地图进行了改进。将多个原子模型与沉积的EM图相关联提供了一种快速且可访问的收敛报告器，这是高分辨率分子细节的强有力指标，并且是对图和模型质量进行FSC独立评估的重要一步。
There does not currently exist a standardized indicator of how well cryo-EM-derived models represent the density from which they were generated. We present a straightforward methodology that utilizes freely available tools to generate a suite of independent models and to evaluate their convergence in an EM density. These analyses provide both a quantitative and qualitative assessment of the precision of the models and their representation of the density, respectively, while concurrently providing a platform for assessing both global and local EM map quality. We further use standardized datasets to provide an expected deviation within a suite of models refined against EM maps reported to be at 5 angstrom resolution or better. Associating multiple atomic models with a deposited EM map provides a rapid and accessible reporter of convergence, a strong indicator of highly resolved molecular detail, and is an important step toward an FSC-independent assessment of map and model quality.