Characterization of a novel autophagy-specific gene, ATG29
Characterization of a novel autophagy-specific gene, ATG29
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DOI：
10.1016/j.bbrc.2005.10.163
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发表时间：
2005-12-30
影响因子：
3.1
通讯作者：
Ohsumi, Y
中科院分区：
文献类型：
--
作者：
Kawamata, T;Kamada, Y;Ohsumi, Y
自噬是细胞质蛋白和细胞器被隔离以在液泡/溶酶体中大量降解的过程。目前，在酿酒酵母中已发现16个ATG基因对自噬体的形成至关重要。这些基因中的大多数也参与细胞质到液泡的运输途径，该途径与自噬共享机制。大多数Atg蛋白共定位于自噬体的前结构（PAS），自噬体被认为起源于此，但自噬的确切机制仍然知之甚少。在旨在获得自噬所需的新基因的遗传筛选过程中，我们鉴定了一个新的ORF，ATG 29/YPL 166 w。atg 29 δ细胞对饥饿敏感，自噬诱导严重延迟。然而，CVT通道运行正常。因此，ATG 29是自噬特异性需要的ATG基因。此外，观察到Atg 29-GFP融合蛋白定位于PAS。从这些结果，我们建议，Atg 29的功能在PAS的自噬体的形成与其他Atg蛋白的合作。(c)2005年爱思唯尔公司All rights reserved.
Autophagy is a process whereby cytoplasmic proteins and organelles are sequestered for bulk degradation in the vacuole/lyso some. At present, 16 ATG genes have been found that are essential for autophagosome formation in the yeast Saccharomyces cerevisiae. Most of these genes are also involved in the cytoplasm to vacuole transport pathway, which shares machinery with autophagy. Most Atg proteins are colocalized at the pre-autophagosomal structure (PAS), from which the autophagosome is thought to originate, but the precise mechanism of autophagy remains poorly understood. During a genetic screen aimed to obtain novel gene(s) required for autophagy, we identified a novel ORF, ATG29/YPL166w. atg29 Delta cells were sensitive to starvation and induction of autophagy was severely retarded. However, the Cvt pathway operated normally. Therefore, ATG29 is an ATG gene specifically required for autophagy. Additionally, an Atg29-GFP fusion protein was observed to localize to the PAS. From these results, we propose that Atg29 functions in autophagosome formation at the PAS in collaboration with other Atg proteins. (c) 2005 Elsevier Inc. All rights reserved.