REGULATION OF GROWTH OF NORMAL OVARIAN EPITHELIAL-CELLS AND OVARIAN-CANCER CELL-LINES BY TRANSFORMING GROWTH-FACTOR-BETA
REGULATION OF GROWTH OF NORMAL OVARIAN EPITHELIAL-CELLS AND OVARIAN-CANCER CELL-LINES BY TRANSFORMING GROWTH-FACTOR-BETA
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DOI：
10.1016/0002-9378(92)91697-9
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发表时间：
1992-02-01
影响因子：
9.8
通讯作者：
BAST, RC
中科院分区：
文献类型：
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作者：
BERCHUCK, A;RODRIGUEZ, G;BAST, RC
目的：这项研究的目的是研究转化生长因子β在调节正常和恶性卵巢上皮细胞增殖中的作用。研究：我们检查了原发性单层培养物中转化生长因子β的产生和反应性，来自五个正常人卵巢和五个卵巢癌细胞系的上皮细胞。分子：在正常的卵巢上皮细胞中，增殖总是被抑制通过转化生长因子β（> 40％）（p <0.01）。 在癌细胞系中，一种显着抑制了一种（> 95％）（p <0.01），其中两个仅受到适度抑制（15％至20％）（p <0.05）（p <0.05），而两个则不受影响。 此外，我们发现所有正常的卵巢上皮细胞和五个卵巢癌细胞系中的四个都会产生转化的生长因子β核糖核酸和蛋白质。结论：这些数据表明，转化生长因子β可以充当自分泌生长抑制作用体内正常卵巢上皮的因子。 因为大多数卵巢癌细胞系对转化生长因子β的生长抑制作用相对抗性，并且一个细胞系不会产生转化的生长因子β，因此可能会导致转化生长因子β途径的损失可能在一些卵巢癌的发展中发挥作用。
OBJECTIVE: The purpose of this study was to study the role of transforming growth factor-beta in regulation of proliferation of normal and malignant ovarian epithelial cells.STUDY DESIGN: We examined production of and responsiveness to transforming growth factor-beta in primary monolayer cultures of epithelial cells from five normal human ovaries and in five ovarian cancer cell lines.RESULTS: In normal ovarian epithelial cells, proliferation always was inhibited by transforming growth factor-beta (> 40%) (p < 0.01). Among the cancer cell lines, proliferation of one was markedly inhibited (> 95%) (p < 0.01), two were only modestly inhibited (15% to 20%) (p < 0.05), and two were unaffected. In addition, we found that all of the normal ovarian epithelial cells and four of five ovarian cancer cell lines produce transforming growth factor-beta ribonucleic acid and protein.CONCLUSIONS: These data suggest that transforming growth factor-beta may act as an autocrine growth inhibitory factor for normal ovarian epithelium in vivo. Because most of the ovarian cancer cell lines are relatively resistant to the growth inhibitory effect of transforming growth factor-beta and because one cell line does not produce transforming growth factor-beta, it is possible that loss of the transforming growth factor-beta pathway may play a role in the development of some ovarian cancers.