Frequent, geographically structured heteroplasmy in the mitochondria of a flowering plant, ribwort plantain (Plantago lanceolata).
Frequent, geographically structured heteroplasmy in the mitochondria of a flowering plant, ribwort plantain (Plantago lanceolata).
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DOI：
10.1038/hdy.2016.15
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发表时间：
2016-07
期刊：
影响因子：
3.8
通讯作者：
Wolff K
中科院分区：
文献类型：
--
作者：
Levsen N;Bergero R;Charlesworth D;Wolff K
最近的研究令人信服地记录了一小部分野生和栽培植物物种中线粒体异质性的案例。异质性被怀疑在开花植物中很常见，对其他分类群的研究可能有助于了解产生异质性的机制及其对植物表型的影响。线粒体异质性的作用在植物中特别令人感兴趣，因为细胞质雄性不育性由线粒体基因型控制，有时会导致雌性和两性个体（gynodiodiopathy）共存。父系泄漏可能是重要的，在这些人口的交配系统的演变。我们进行了一项遗传调查的雌株异株植物车前草，其中异质性以前没有报道，并估计线粒体基因型和异质性的频率。桑格序列基因分型的179个人从15个欧洲人口的两个多态性线粒体位点，atp 6和rps 12，确定了15个异质性的个人。这些分布在10个群体中的6个群体中，这些群体在目标基因座中具有多态性，占所有采样个体的8%，占这6个群体中个体的15%。北方英格兰和苏格兰的发病率最高。我们的研究结果是一致的地理差异的发生率父系泄漏和/或核恢复男性生育力。
Recent research has convincingly documented cases of mitochondrial heteroplasmy in a small set of wild and cultivated plant species. Heteroplasmy is suspected to be common in flowering plants and investigations of additional taxa may help understand the mechanisms generating heteroplasmy as well as its effects on plant phenotypes. The role of mitochondrial heteroplasmy is of particular interest in plants as cytoplasmic male sterility is controlled by mitochondrial genotypes, sometimes leading to co-occurring female and hermaphroditic individuals (gynodioecy). Paternal leakage may be important in the evolution of mating systems in such populations. We conducted a genetic survey of the gynodioecious plant Plantago lanceolata, in which heteroplasmy has not previously been reported, and estimated the frequencies of mitochondrial genotypes and heteroplasmy. Sanger sequence genotyping of 179 individuals from 15 European populations for two polymorphic mitochondrial loci, atp6 and rps12, identified 15 heteroplasmic individuals. These were distributed among 6 of the 10 populations that had polymorphisms in the target loci and represented 8% of all sampled individuals and 15% of the individuals in those 6 populations. The incidence was highest in Northern England and Scotland. Our results are consistent with geographic differences in the incidence of paternal leakage and/or the rates of nuclear restoration of male fertility.