Three-dimensional microarray compared with PCR-single-strand conformation polymorphism analysis/DNA sequencing for mutation analysis of K-ras codons 12 and 13
Three-dimensional microarray compared with PCR-single-strand conformation polymorphism analysis/DNA sequencing for mutation analysis of K-ras codons 12 and 13
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DOI：
10.1373/clinchem.2004.032060
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发表时间：
2004-08-01
影响因子：
9.3
通讯作者：
Kanno, T
中科院分区：
文献类型：
--
作者：
Maekawa, M;Nagaoka, T;Kanno, T
背景资料：我们开发了一种快速、精确和准确的基于微阵列的方法，该方法使用三维平台检测突变。方法：我们使用PamChip（R）微阵列检测15个细胞系和81个胃癌或结直肠癌组织中K-ras密码子12和13的突变。异硫氰酸黄绿素标记的PCR产物用微阵列分析。结果：PamChip基因芯片可准确识别野生型、杂合型和纯合型突变基因型，并可用于临床诊断。
Background: We developed a rapid, precise, and accurate microarray-based method that uses a three-dimensional platform for detection of mutations.Methods: We used the PamChip(R) microarray to detect mutations in codons 12 and 13 of K-ras in 15 cell lines and 81 gastric or colorectal cancer tissues. Fluorescein isothiocyanate-labeled PCR products were analyzed with the microarray. We confirmed the microarray results with PCR-single-strand conformation polymorphism (SSCP) analysis and DNA sequencing.Results: We could correctly identify wild-type, heterozygous, and homozygous mutant genotypes with the PamChip microarray in