c-Kit immunoreactive interstitial cells in the human gastrointestinal tract
c-Kit immunoreactive interstitial cells in the human gastrointestinal tract
复制标题
DOI：
10.1016/s0165-1838(98)00174-x
复制
发表时间：
1999-01-15
期刊：
影响因子：
--
通讯作者：
Watanabe, Y
中科院分区：
文献类型：
--
作者：
Torihashi, S;Horisawa, M;Watanabe, Y
c-Kit免疫阳性细胞被认为是胃肠道中神经传递的起搏器和/或介质。它们也对应于小鼠中的Cajal间质细胞（IC）。c-Kit阳性细胞在人体胃肠道的正常分布及其与IC的关系尚不清楚。在这项研究中，我们研究了c-Kit阳性细胞的分布和它们的超微结构在正常人体组织。然后，我们对它们进行分类，并研究它们与IC的关系。对39例人（年龄从16周龄胎儿到57岁，无运动障碍）从食管到乙状结肠的肠道样品进行免疫组织化学、电子显微镜和免疫电子显微镜检查。c-Kit免疫阳性细胞位于从食管下段到乙状结肠的由平滑肌细胞组成的外肌中，它们在形态学上分为两组。第一组细胞主要为梭形双极细胞，分支较少，这些细胞与附近的平滑肌平行。超微结构上，它们具有许多中间丝和小窝。梭形细胞分布于食管、胃和小肠。第二组细胞仅位于结肠内，是具有许多分支的多极或双极细胞。第二组细胞也富含小窝和/或滑面内质网，但中间丝不明显。虽然两组c-Kit免疫阳性细胞对应于IC，但人肠道中的一些IC似乎不表达c-Kit免疫反应性。(C)1999 Elsevier Science B. V.保留所有权利。
c-Kit immunopositive cells are considered to be pacemakers and/or mediators of neurotransmission in the gastrointestinal tract. They also correspond to the interstitial cells of Cajal (ICs) in mice. The normal distribution of c-Kit positive cells and their relation to ICs in the human gastrointestinal tract remain unclear. In this study we examine the distribution of c-Kit positive cells and their ultrastructure in normal human tissue. We then classified them and examined their relationship to ICs. Thirty nine samples of gut from the esophagus to the sigmoid colon from humans (ranging in age from a 16 week old fetus to a 57 year old and without motility disorders), were processed for immunohistochemistry, electronmicroscopy and immuno-electronmicroscopy. c-Kit immunopositive cells were located in the external muscle from the lower esophagus to the sigmoid colon, wherever the external muscle was composed of smooth muscle cells, and they were classified morphologically into two groups. Cells in the first group were mainly spindle-shaped bipolar cells with few branches; these cells ran parallel to nearby smooth muscle. Ultrastructurally, they possessed many intermediate filaments and caveolae. The spindle-shaped cells were present in the esophagus, stomach and small intestine. The second group of cells were located only in the colon, and were multipolar or bipolar cells with numerous branches. Cells in the second group were also rich in caveolae and/or smooth endoplasmic reticulum, but intermediate filaments were not prominent. Although both groups of c-Kit immunopositive cells corresponded to ICs, some ICs in the human gut do not appear to express c-Kit immunoreactivity. (C) 1999 Elsevier Science B.V. All rights reserved.