SELECTIVE NEURONAL GLYCOCONJUGATE EXPRESSION IN SENSORY AND AUTONOMIC GANGLIA - RELATION OF LECTIN REACTIVITY TO PEPTIDE AND ENZYME MARKERS
SELECTIVE NEURONAL GLYCOCONJUGATE EXPRESSION IN SENSORY AND AUTONOMIC GANGLIA - RELATION OF LECTIN REACTIVITY TO PEPTIDE AND ENZYME MARKERS
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DOI：
10.1007/bf01188046
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发表时间：
1990-10-01
期刊：
影响因子：
--
通讯作者：
KRUGER, L
中科院分区：
文献类型：
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作者：
SILVERMAN, JD;KRUGER, L
几种植物凝集素被用来表征细胞表面的碳水化合物上的感觉神经节细胞和它们的中央终末在脊髓背角。在大鼠中，在其中央轴突投射到胶状质的小神经元的大亚群上的半乳糖末端糖缀合物用α-半乳糖末端糖缀合物来证明。D-半乳糖特异性Griffonia simplicifolia I-B4（GSA）凝集素。该神经元亚群用交替的D-半乳糖-、N-乙酰半乳糖胺-和β Gal（1，3）NAcGal-结合凝集素标记。在选定的外周自主神经、味觉和内脏感觉和肠神经元以及副嗅球中也表现出类似的GSA凝集素反应性。感觉神经元特异性同工酶，耐氟酸性磷酸酶（FRAP）与GSA凝集素共定位，单克隆抗体（MAb）2C 5也是如此，该抗体针对构成ABH人血型抗原骨架结构的乳糖系列碳水化合物。相反，降钙素基因相关肽免疫反应性（CGRP-IR），作为肽能神经元的代表性标志物，表现出有限的共定位与GSA。多克隆抗大鼠红细胞（RBC）抗体与GSA共定位，表明大鼠感觉神经元上的凝集素反应性碳水化合物与大鼠RBC抗原有关。在人脊髓中，L-果糖结合荆豆-I（UEA）凝集素也标记胶状质;在兔中，一个小的感觉神经节细胞亚群和脊髓胶状质共同标记的GSA和UEA凝集素。这些研究说明了大鼠非肽能FRAP（+）感觉神经节细胞的显著凝集素反应性细胞表面碳水化合物表达，并提供了一种可视化广泛的非肽能小感觉神经节细胞亚群的方法，可能包括相当大比例的伤害性和无髓鞘外周轴突。
Several plant lectins were used to characterize the cell-surface carbohydrates expressed on sensory ganglion cells and their central terminals in the spinal cord dorsal horn. In the rat, galactose-terminal glycoconjugates on a large subpopulation of small neurons whose central axons project to the substantia gelatinosa were demonstrated with the .alpha.-D-galactose-specific Griffonia simplicifolia I-B4 (GSA) lectin. This neuron subset was labelled by alternative D-galactose-, N-Acetylgalactosamine-, and .beta.Gal(1,3)NAcGal-binding lectins. Similar GSA lectin reactivity was also illustrated in selected peripheral autonomic, gustatory and visceral sensory and enteric neurons, and the accessory olfactory bulb. The sensory neuron-specific isoenzyme, fluoride-resistant acid phosphatase (FRAP) co-localized with the GSA lectin, as did the monoclonal antibody (MAb) 2C5, which is directed against a lactoseries carbohydrate constituting a backbone structure of ABH human blood group antigens. In contrast, calcitonin gene-related peptide-immunoreactivity (CGRP-IR), used as a representative marker of peptidergic neurons, exhibited limited co-localization with GSA. A polyclonal anti-rat red blood cell (RBC) antibody co-localized with GSA, suggesting that lectin-reactive carbohydrates on rat sensory neurons are related to rat RBC antigens. In the human spinal cord, the L-frucose-binding Ulex europaeus-I (UEA) lectin also labelled the substantia gelatinosa; in rabbit, a small sensory ganglion cell subset and the spinal cord substantia gelatinosa was co-labelled by both the GSA and UEA lectins. These studies illustrate significant lectin-reactive cell surface carbohydrate expression by non-peptidergic, FRAP(+) sensory ganglion cells in the rat, and provide a means for visualizing the extensive, non-peptidergic, small sensory ganglion cell subpopulations, probably including a substantial proportion of nociceptive and unmyelinated peripheral axons.