Isolation and characterization of the products of anoxic irradiation of d(CpGpTpA)
Isolation and characterization of the products of anoxic irradiation of d(CpGpTpA)
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DOI：
10.1080/095530097144210
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发表时间：
1997-03-01
影响因子：
2.6
通讯作者：
Box, HC
中科院分区：
文献类型：
--
作者：
Budzinski, EE;Dawidzik, JB;Box, HC
提出了一种分离DNA低聚物水溶液辐照产物的改进方法。从四聚体d（CpGpTpA）中分离得到39个产物。其中，16个，包括大多数主要产品，是通过使用质子核磁共振波谱鉴定的。鉴定的产物分为四类：(1)碱基产物，(2)链断裂产物，(3)碱基修饰和(4)串联损伤。胸腺嘧啶甲基碳原子与鸟嘌呤C8碳原子共价连接的串联损伤比任何简单的碱基修饰的产率都高。
An improved method for separating the products of DNA oligomers irradiated in aqueous solution has been devised. Altogether 39 products were isolated from the tetramer d(CpGpTpA) X-irradiated in dilute anoxic solution. Of these, 16, including most of the major products, were identified through the use of proton nmr spectroscopy. The identified products fall into four categories: (1) base products, (2) strand scission products, (3) base modifications and (4) tandem lesion. A tandem lesion in which the methyl carbon atom of thymine is covalently linked to the guanine C8 carbon atom was produced in larger yield than any of the simple base modifications.