In vitro generation of an osteochondral interface from mesenchymal stem cell-collagen microspheres
In vitro generation of an osteochondral interface from mesenchymal stem cell-collagen microspheres
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DOI：
10.1016/j.biomaterials.2010.10.021
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发表时间：
2011-02-01
期刊：
影响因子：
14
通讯作者：
Chan, Barbara P.
中科院分区：
文献类型：
--
作者：
Cheng, Hiu-Wa;Luk, Keith D. K.;Chan, Barbara P.
在机械结构不同的组织之间建立生物界面是复杂组织工程中的一个关键挑战。骨软骨界面对于防止机械破坏和维持软骨的正常功能是必不可少的。尽管在开发骨软骨塞方面做出了巨大的努力，但尚未有形成具有适当带状组织的骨软骨界面的报道。在这里，我们提出了一种基于间充质干细胞胶原微球的复杂组织工程方法，并在体外形成了干细胞来源的骨软骨界面，钙化软骨界面分隔了未钙化的软骨层和下面的骨层。交界区的细胞是肥大的软骨细胞，而该区域的细胞外基质含有11型和X型胶原、钙沉积和垂直排列的纤维。在共培养过程中，适当的介质和配置的同时存在对于界面的形成是必要的。(C)2010爱思唯尔有限公司。保留所有权利。
Creating biological interfaces between mechanically dissimilar tissues is a key challenge in complex tissue engineering. An osteochondral interface is essential in preventing mechanical failure and maintaining normal function of cartilage. Despite tremendous efforts in developing osteochondral plugs, formation of the osteochondral interface with proper zonal organization has not yet been reported. Here, we present a mesenchymal stem cell collagen microsphere-based approach for complex tissue engineering and demonstrate in vitro formation of a stem cell-derived osteochondral interface with calcified cartilage interface separating a non-calcified cartilage layer and an underlying bone layer. Cells at the interface region are hypertrophic chondrocytes while the extracellular matrix in this region contains collagen type 11 and X, calcium deposits and vertically running fibers. The simultaneous presence of appropriate medium and configuration during co-culture is necessary for the interface formation. (C) 2010 Elsevier Ltd. All rights reserved.