Facile synthesis of the nitrogen-doped graphene quantum dots at low temperature for cellular labeling
Facile synthesis of the nitrogen-doped graphene quantum dots at low temperature for cellular labeling
复制标题
低温下轻松合成氮掺杂石墨烯量子点用于细胞标记
DOI：
10.1016/j.materresbull.2018.03.053
复制
发表时间：
2018-08-01
影响因子：
5.4
通讯作者：
Li, Xiaolong
中科院分区：
文献类型：
--
作者：
Pang, Yuqian;Gao, Hui;Li, Xiaolong
以柠檬酸（CA）为前驱体，采用一步法在低温下大规模合成了氮掺杂石墨烯量子点（N-GQD）。N-GQD的发射波长几乎是激发依赖性的，这可以反映来自不同尺寸的颗粒和纳米颗粒上不同发射位点的分布的影响。此外，这些产品还通过活的Hela细胞进行测试，以获得非凡的生物相容性。即使在暴露于200 μ g/mL（-1）的高浓度N-GQD 48小时后，细胞存活率仍保持为100%，并且该存活率实际上远高于先前报道的存活率。所制备的N-GQD具有可忽略的固有毒性，显示出优异的生物应用，如生物成像和细胞追踪。
A facile one-step approach is developed for the large-scale synthesis of nitrogen doped graphene quantum dot (N-GQDs) at low temperature by using citric acid (CA) as the precursor. The emission wavelength of the N-GQDs is nearly excitation-dependent, which may mirror the effects from particles of different sizes and distribution of different emissive sites on nanoparticles. Moreover, the products are then tested with live Hela cells for the extraordinary biocompatibility. The cellular viability is remained to be 100% even after a 48 h exposure with the high N-GQDs concentration of 200 mu g mL(-1) and this survival rate is really much higher than that have been repotted previously. The as-prepared N-GQDs with negligible inherent toxicity exhibit excellent biological applications such as bio-imaging and cell tracking.