Removal of Qa-2 antigen alters the Ped gene phenotype of preimplantation mouse embryos.
Removal of Qa-2 antigen alters the Ped gene phenotype of preimplantation mouse embryos.
复制标题
Qa-2 抗原的去除会改变植入前小鼠胚胎的 Ped 基因表型。
DOI：
10.1095/biolreprod47.2.271
复制
发表时间：
1992
影响因子：
3.6
通讯作者：
Warner,CM
中科院分区：
文献类型：
--
作者：
Tian,Z;Xu,Y;Warner,CM
胚胎的存活受到遗传和环境因素的影响。我们实验室以前的研究已经确定了一个与胚胎存活相关的基因，Pedgene，一个与小鼠主要组织相容性复合体（MHC）相关的基因。Pedgene已被证明影响植入前胚胎卵裂分裂率，以及窝仔数，出生体重和断奶体重。Pedgene的遗传作图已将其定位于MHC的Q区，并提示编码Pedgene的可能的Q区基因是Q3、Q5、Q6、Q7、Q8和/或Q9。尽管Q3和Q5基因的蛋白产物是未知的，但非常相似的Q6、Q7、Q8和Q9基因的蛋白产物是Qa-2抗原。已知两种形式的膜结合Qa-2抗原：糖基磷脂酰肌醇（GPI）连接的和跨膜结合的。只有GPI连接的形式对磷脂酰肌醇磷脂酶C（PI-PLC）的裂解敏感。本研究的第一个目的是确定Qa-2抗原与植入前小鼠胚胎细胞表面连接的性质。发现胚胎细胞表面上所有可检测的Qa-2抗原对PI-PLC的切割敏感，因此通过GPI连接与细胞膜结合。此外，从胚胎细胞表面去除Qa-2抗原减缓了植入前小鼠胚胎的发育速率。这些结果表明Qa-2抗原可能是Pedgene产物。
Embryo survival is influenced by both genetic and environmental factors. Previous research in our laboratory has identified one gene associated with embryonic survival, thePedgene, a gene that is linked to the major histocompatibility complex (MHC) of the mouse. ThePedgene has been shown to influence the rate of preimplantation embryonic cleavage division, as well as litter size, birth weight, and weaning weight. Genetic mapping of thePedgene has located it in the Q region of the MHC and has suggested that possible Q region genes encoding thePedgene are Q3, Q5, Q6, Q7, Q8, and/or Q9. Whereas the protein products of the Q3 and Q5 genes are unknown, the protein product of the very similar Q6, Q7, Q8, and Q9 genes is the Qa-2 antigen. Two forms of membrane-bound Qa-2 antigen are known: glycosylphosphatidylinositol (GPI)-linked and transmembrane bound. Only the GPI-linked form is sensitive to cleavage by phosphatidylinositol phospholipase C (PI-PLC). The first purpose of the present study was to determine the nature of the linkage of the Qa-2 antigen to the cell surface of preimplantation mouse embryos. It was found that all detectable Qa-2 antigen on the embryonic cell surface is sensitive to cleavage by PI-PLC and is therefore bound to the cell membrane by a GPI linkage. Furthermore, removal of Qa-2 antigen from the embryonic cell surface slows down the rate of development of preimplantation mouse embryos. These results suggest the likelihood that the Qa-2 antigen is thePedgene product.