ANTITUMOR IMMUNITY INDUCED BY PHOTODYNAMIC THERAPY WITH ALUMINUM DISULFONATED PHTHALOCYANINES AND LASER-LIGHT
ANTITUMOR IMMUNITY INDUCED BY PHOTODYNAMIC THERAPY WITH ALUMINUM DISULFONATED PHTHALOCYANINES AND LASER-LIGHT
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DOI：
10.1097/00001813-199408000-00009
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发表时间：
1994-08-01
影响因子：
2.3
通讯作者：
CUBEDDU, R
中科院分区：
文献类型：
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作者：
CANTI, G;LATTUADA, D;CUBEDDU, R
光动力疗法（PDT）是全身给药肿瘤局部光敏剂，随后用适当波长的光照射。恶性组织的药物摄取与激光产生的光的选择性传递相结合提供了有效的治疗方法，具有有效的肿瘤细胞毒性和最小的正常组织损伤。目前关于光活化光敏剂对宿主免疫反应影响的研究较少。由于免疫在控制肿瘤生长和扩散方面很重要，我们在实验室中研究了光活化酞菁对抗肿瘤免疫反应的影响。用5 mg/kg二磺酸酞菁铝（AIS2Pc）治疗免疫抑制小鼠和正常小鼠的MS-2纤维肉瘤，然后将肿瘤块暴露于激光（100 mW/cm2 × 10 min）或手术切除肿瘤，两组间无差异。在上述治疗方式后无肿瘤100天的幸存者再次接受母体MS-2检查。部分存活动物在注射肿瘤前被环磷酰胺免疫抑制。仅PDT治愈的正常存活动物对再攻击有抵抗，而免疫抑制存活动物和手术治愈的动物则死于肿瘤。最后，用PDT治愈且无肿瘤的小鼠，再用L1210和P388小鼠白血病攻击，没有存活。这些结果表明，光激活AIS2Pc的PDT可诱导潜在的特异性“抗肿瘤免疫”。
Photodynamic therapy (PDT) is systemic administration of tumor localizing photosensitizers and subsequent irradiation with light of the appropriate wavelength. The combination of drug uptake in malignant tissues and selective delivery of laser-generated light provides effective therapy, with efficient tumor cytotoxicity and minimal normal tissue damage. There have been few studies of the effects of photoactivated photosensitizers on the host immune response. Since immunity is important in the control of tumor growth and spread, we have examined, in our laboratory, the effects of photoactivated phthalocyanines on the antitumor immune response. Immunosuppressed and normal mice bearing the MS-2 fibrosarcoma treated with 5 mg/kg of aluminum disulfonated phthalocyanine (AIS2Pc) and then the tumor mass exposed to laser light (100 mW/cm2 x 10 min) or treated with surgical excision of the tumor survived indefinitely, with no difference between the different groups. The survivors, tumor-free 100 days after the treatment modalities described above, were rechallenged with the parental MS-2. Some groups of surviving animals were immunosuppressed with cyclophosphamide before the injection of the tumor. Resistance to rechallenge was evidenced only in normal surviving animals cured by PDT, while the immunodepressed surviving animals and animals cured by surgery died of tumor. Finally, mice, cured by PDT and tumor-free, rechallenged with L1210 and P388 murine leukemias did not survive. These results suggest that a potential and specific 'antitumor immunity' is induced by PDT with photoactivated AIS2Pc.