Supercritical fluid extraction of phenol compounds from olive leaves
Supercritical fluid extraction of phenol compounds from olive leaves
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DOI：
10.1016/s0039-9140(97)00375-5
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发表时间：
1998-08-01
期刊：
影响因子：
6.1
通讯作者：
Valcarcel, M
中科院分区：
文献类型：
--
作者：
Le Floch, F;Tena, MT;Valcarcel, M
研究了一种清洁的、高选择性的超临界流体萃取（SFE）方法从橄榄叶样品中分离酚类物质。使用Folin-Ciocalteu试剂测定总酚浸提物。使用用10%甲醇改性的二氧化碳在334巴下对干燥、研磨、过筛的橄榄叶样品（30 mg）进行SFE。100 ℃（CO2密度0.70 g ml（-1）），液体流速2 ml min（-1），持续140 min。使用硅藻土减少萃取容器的空隙体积。研究了改性剂含量、压力、温度、流速、萃取时间和收集/洗脱变量等萃取变量的影响。使用电喷雾-MS（负离子模式）筛选超临界流体提取物中的酸化合物，如羧酸和酚类。在正己烷、乙醚和乙酸乙酯等液体溶剂中，超临界萃取的苯酚回收率高于超声处理，但用液体甲醇时，苯酚的提取率仅为45%。(C)1998 Elsevier Science B. V.保留所有权利。
A clean, highly selective supercritical fluid extraction (SFE) method for the isolation of phenols from olive leaf samples was examined. Total phenol extracts were determined using the Folin-Ciocalteu reagent. Dried, ground, sieved olive leaf samples (30 mg) are subjected to SFE, using carbon dioxide modified with 10% methanol at 334 bar. 100 degrees C (CO2 density 0.70 g ml(-1)) at a liquid flow-rate of 2 ml min(-1) for 140 min. Diatomaceous earth is used to reduce the void volume of the extraction vessel. The influence of extraction variables such as modifier content, pressure, temperature, flow-rate, extraction time, and collection/elution variables, were studied. Supercritical fluid extracts were screened for acid compounds such as carboxylic acids and phenols using Electrospray-MS (in the negative ionization mode). SFE was found to produce higher phenol recoveries than sonication in liquid solvents such as nehexane, diethyl ether and ethyl acetate, However, the extraction yield obtained was only 45%, using liquid methanol. (C) 1998 Elsevier Science B.V. All rights reserved.