Lariatins, novel anti-mycobacterial peptides with a lasso structure, produced by Rhodococcus jostii K01-B0171
Lariatins, novel anti-mycobacterial peptides with a lasso structure, produced by Rhodococcus jostii K01-B0171
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DOI：
10.1038/ja.2007.48
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发表时间：
2007-06-01
影响因子：
3.3
通讯作者：
Omura, Satoshi
中科院分区：
文献类型：
--
作者：
Iwatsuki, Masato;Uchida, Ryuji;Omura, Satoshi
从云南土壤团聚体中分离到的贾氏红球菌K 01-B 0171菌株发酵液中，经HP-20柱层析和ODS柱层析分离得到两种具有套索结构的抗分枝杆菌肽，命名为Lariatins A和B。琼脂稀释法测得Lariains A和B对耻垢分枝杆菌的MIC值分别为3.13和6.25 μ g/ml。液体微量稀释法测得其对结核分枝杆菌的MIC为0.39 μ g/ml。
Two anti-mycobacterial peptides with a lasso structure, named lariatins A and B, were separated by HP-20 and ODS column chromatographies and purified by HPLC from the culture broth of Rhodococcus jostii K01-B0171, which was isolated from soil aggregates collected in Yunnan, China. Lariains A and B showed growth inhibition against Mycobacterium smegmatis with MIC values of 3.13 and 6.25 mu g/ml in agar dilution method, respectively. Furthermore, lariatin A inhibited the growth of Mycobacterium tuberculosis with an MIC of 0.39 mu g/ml in liquid microdilution method.